We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.

中文翻译：

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使用 Spotiton 的时间分辨冷冻电镜。

我们提出了一种制备低温电子显微镜 (cryo-EM) 网格以研究短寿命分子状态的方法。使用压电分配，两个独立的 ~50 pl 样品液滴流在 10 毫秒内沉积到纳米线 EM 网格的表面上，当网格在液体乙烷中玻璃化约 100 毫秒后，混合反应停止。我们为四种生物系统展示了这种方法，其中短命状态非常受关注。