ABSTRACT Functional genomics can be applied to shed light on the Brassica napus – Verticillium interaction. RNAseq data indicated already that abscisic acid (ABA) is apparently involved in the early oilseed rape response to fungal infection with Verticillium longisporum isolate 43 (Vl43). A calreticulin (CRT1a) was identified as novel susceptibility factor for Vl43 infecting both Arabidopsis and oilseed rape. CRT1a is involved in calcium homeostasis and contributes in the endoplasmatic reticulum to the unfolded protein response. The latter function could either affect the correct folding of other susceptibility factors or of negative regulators in ethylene (ET) signaling. Which CRT1a function is affected in the mutants is currently unknown, but both hypotheses can explain that crt1a loss-of-function mutants display increased resistance to V. longisporum and enhanced expression of ethylene signaling related genes. This indicates that besides other phytohormones such as ABA or salicylic acid (SA) also ET plays a critical role in the plant-Verticillium interaction, which might be exploited to improve plant resistance.

中文翻译：

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宿主-轮枝菌相互作用中的植物激素串扰

摘要 功能基因组学可用于阐明欧洲油菜与轮枝菌的相互作用。RNAseq 数据已经表明脱落酸 (ABA) 显然参与了油菜对长孢轮枝菌分离株 43 (Vl43) 真菌感染的早期反应。钙网蛋白 (CRT1a) 被鉴定为 Vl43 感染拟南芥和油菜的新型易感因子。CRT1a 参与钙稳态并在内质网中参与未折叠蛋白反应。后一种功能可能会影响其他易感因素或乙烯 (ET) 信号中负调节因子的正确折叠。目前尚不清楚突变体中哪些 CRT1a 功能受到影响，但两种假设都可以解释 crt1a 功能丧失突变体对 V 的抗性增加。长孢子菌和乙烯信号相关基因的增强表达。这表明除了其他植物激素如 ABA 或水杨酸 (SA) 外，ET 在植物-轮枝菌相互作用中也起着关键作用，可用于提高植物抗性。