ABSTRACT

Introduction

Autophagy is an evolutionarily conserved cellular clearance process, by which cytosolic components are delivered to autolysosomes for breakdown and recycling to maintain cellular homeostasis. During the past decades, autophagy has been found to be tightly implicated in various physiological and pathological progresses. Unraveling the regulatory mechanisms of the autophagy process will contribute to the development of emerging autophagy-targeting strategies for the treatment of various diseases. Recently, the rapid development of proteomics approaches has enabled the use of large-scale unbiased strategies to unravel autophagy machinery.

Areas covered

In this review, we will highlight the recent contributions of proteomics strategies in clarifying the autophagy machinery, with an emphasis on the three different types of autophagy (namely macroautophagy, microautophagy, and chaperone-mediated autophagy). We will also discuss the emerging role of proteomics approaches in investigating the mechanism of the autophagy-based unconventional secretory pathway (secretory autophagy).

Expert opinion

Proteomics has provided an effective strategy for the comprehensive analysis of the autophagy process, which will broaden our understanding of autophagy machinery, and holds great promise for developing clinical therapies targeting autophagy.

中文翻译：

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自噬机制的最新进展：蛋白质组学观点。

摘要

介绍

自噬是一种进化上保守的细胞清除过程，通过该过程，细胞溶质成分被输送到自溶酶体进行分解和回收，以维持细胞稳态。在过去的几十年中，发现自噬与各种生理和病理进展密切相关。解开自噬过程的调控机制将有助于开发用于治疗各种疾病的新兴自噬靶向策略。最近，蛋白质组学方法的快速发展使得能够使用大规模的无偏策略来解开自噬机制。

覆盖区域

在这篇综述中，我们将重点介绍蛋白质组学策略在阐明自噬机制方面的最新贡献，重点是三种不同类型的自噬（即巨自噬、微自噬和分子伴侣介导的自噬）。我们还将讨论蛋白质组学方法在研究基于自噬的非常规分泌途径（分泌自噬）机制方面的新兴作用。

专家意见

蛋白质组学为全面分析自噬过程提供了一种有效的策略，将拓宽我们对自噬机制的理解，并为开发针对自噬的临床疗法提供广阔前景。