Abstract Bacteriocins are small peptides that can inhibit the growth of a diverse range of microbes. There is a need to identify bacteriocins that are effective against biofilms of resistant clinical strains. The present study focussed on the efficacy of purified nisin like bacteriocin-GAM217 against extended spectrum β-lactamase (ESBL) and metallo-beta-lactamase (MBL) producing clinical strains. Bacteriocin-GAM217 when combined with curcumin and cinnamaldehyde, synergistically enhanced antibacterial activity against planktonic and biofilm cultures of Staphylococcus epidermidis and Escherichia coli. Bacteriocin-GAM217 and phytochemical combinations inhibited biofilm formation by >80%, and disrupted the biofilm for selected ESBL and MBL producing clinical strains. The anti-adhesion assay showed that these combinatorial compounds significantly lowered the attachment of bacteria to Vero cells and that they elicited membrane permeability and rapid killing as viewed by confocal microscopy. This study demonstrates that bacteriocin-GAM217 in combination with phytochemicals can be a potential anti-biofilm agent and thus has potential for biomedical applications.

中文翻译：

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乳链菌肽样细菌素与姜黄素和肉桂醛对产生 ESBL 和 MBL 的临床菌株的协同抗菌和抗生物膜活性

摘要 细菌素是一种小肽，可以抑制多种微生物的生长。需要鉴定对临床耐药菌株的生物膜有效的细菌素。本研究的重点是纯化的乳链菌肽类细菌素-GAM217 对产生超广谱 β-内酰胺酶 (ESBL) 和金属β-内酰胺酶 (MBL) 的临床菌株的功效。细菌素-GAM217 与姜黄素和肉桂醛联合使用时，可协同增强对表皮葡萄球菌和大肠杆菌的浮游生物和生物膜培养物的抗菌活性。细菌素-GAM217 和植物化学组合可抑制生物膜形成 > 80%，并破坏选定的产生 ESBL 和 MBL 的临床菌株的生物膜。抗粘连试验表明，这些组合化合物显着降低了细菌对 Vero 细胞的附着，并且在共聚焦显微镜下观察到它们引起膜渗透性和快速杀灭。这项研究表明，细菌素-GAM217 与植物化学物质结合可以成为潜在的抗生物膜剂，因此具有生物医学应用的潜力。