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In vitro biological activity of Hydroclathrus clathratus and its use as an extracellular bioreductant for silver nanoparticle formation
Green Processing and Synthesis ( IF 3.8 ) Pub Date : 2020-08-07 , DOI: 10.1515/gps-2020-0043
Raghad R. Alzahrani 1 , Manal M. Alkhulaifi 1 , Nouf M. Al-Enazi 2
Affiliation  

Abstract The adaptive nature of algae results in producing unique chemical components that are gaining attention due to their efficiency in many fields and abundance. In this study, we screened the phytochemicals from the brown alga Hydroclathrus clathratus and tested its ability to produce silver nanoparticles (AgNPs) extracellularly for the first time. Lastly, we investigated its biological activity against a variety of bacteria. The biosynthesized nanoparticles were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and energy-dispersive spectroscopy. The biological efficacy of AgNPs was tested against eighteen different bacteria, including seven multidrug-resistant bacteria. Phytochemical screening of the alga revealed the presence of saturated and unsaturated fatty acids, sugars, carboxylic acid derivatives, triterpenoids, steroids, and other components. Formed AgNPs were stable and ranged in size between 7 and 83 nm and presented a variety of shapes. Acinetobacter baumannii, Staphylococcus aureus, Methicillin-resistant S. aureus (MRSA), and MDR A. baumannii were the most affected among the bacteria. The biofilm formation and development assay presented a noteworthy activity against MRSA, with an inhibition percentage of 99%. Acknowledging the future of nano-antibiotics encourages scientists to explore and enhance their potency, notably if they were obtained using green, rapid, and efficient methods.

中文翻译:

Hydroclathrus clathratus 的体外生物活性及其作为银纳米颗粒形成的细胞外生物还原剂的用途

摘要 藻类的适应性导致产生独特的化学成分,这些化学成分因其在许多领域的效率和丰度而受到关注。在这项研究中,我们从褐藻 Hydroclathrus clathratus 中筛选了植物化学物质,并首次测试了其在细胞外产生银纳米粒子 (AgNPs) 的能力。最后,我们研究了它对多种细菌的生物活性。生物合成的纳米粒子通过紫外-可见光谱、傅里叶变换红外光谱、动态光散射、透射电子显微镜和能量色散光谱进行表征。AgNPs 的生物学功效针对 18 种不同的细菌进行了测试,其中包括 7 种耐多药细菌。藻类的植物化学筛选显示存在饱和和不饱和脂肪酸、糖、羧酸衍生物、三萜类化合物、类固醇和其他成分。形成的 AgNPs 是稳定的,大小在 7 到 83 nm 之间,并呈现出各种形状。鲍曼不动杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌 (MRSA) 和耐多药鲍曼不动杆菌在这些细菌中受影响最大。生物膜形成和发展试验对 MRSA 具有显着的活性,抑制率为 99%。承认纳米抗生素的未来会鼓励科学家探索和增强它们的效力,特别是如果它们是使用绿色、快速和有效的方法获得的。形成的 AgNPs 是稳定的,大小在 7 到 83 nm 之间,并呈现出各种形状。鲍曼不动杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌 (MRSA) 和耐多药鲍曼不动杆菌在这些细菌中受影响最大。生物膜形成和发展试验对 MRSA 具有显着的活性,抑制率为 99%。承认纳米抗生素的未来会鼓励科学家探索和增强它们的效力,特别是如果它们是使用绿色、快速和有效的方法获得的。形成的 AgNPs 是稳定的,大小在 7 到 83 nm 之间,并呈现出各种形状。鲍曼不动杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌 (MRSA) 和耐多药鲍曼不动杆菌在这些细菌中受影响最大。生物膜形成和发展试验对 MRSA 具有显着的活性,抑制率为 99%。承认纳米抗生素的未来会鼓励科学家探索和增强它们的效力,特别是如果它们是使用绿色、快速和有效的方法获得的。生物膜形成和发展试验对 MRSA 具有显着的活性,抑制率为 99%。承认纳米抗生素的未来会鼓励科学家探索和增强它们的效力,特别是如果它们是使用绿色、快速和有效的方法获得的。生物膜形成和发展试验对 MRSA 具有显着的活性,抑制率为 99%。承认纳米抗生素的未来会鼓励科学家探索和增强它们的效力,特别是如果它们是使用绿色、快速和有效的方法获得的。
更新日期:2020-08-07
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