Aflatoxin M1 (AFM1), a group I carcinogen, can be found in the milk of animals fed with aflatoxin‐B₁‐contaminated feedstuff. In this study, we developed and validated a lateral flow immunoassay (LFIA) with the cut‐off value of 0.5 µg/L for AFM1 screening. The validation revealed that the assay yielded high sensitivity, selectivity and efficiency with no false positives. Forty‐one commercial milk samples were tested for AFM1 by the developed LFIA. However, the developed LFIA could not be applied to milk samples flavoured with chocolate and coffee. Otherwise, all samples gave negative test results.

中文翻译：

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侧向流免疫测定法的开发和验证，用于检测原料奶和商品奶中的黄曲霉毒素M1

黄曲霉毒素M1（AFM1）是一种I类致癌物，存在于被黄曲霉毒素B ₁污染的饲料喂养的动物的牛奶中。在本研究中，我们开发并验证了用于AFM1筛查的临界值为0.5 µg / L的侧向流免疫分析（LFIA）。验证表明，该检测方法具有高灵敏度，选择性和高效率，没有假阳性。发达的LFIA对41个商业牛奶样品进行了AFM1测试。但是，已开发的LFIA无法应用于以巧克力和咖啡调味的牛奶样品。否则，所有样品均给出阴性测试结果。