The morphological and physiological basis of delayed pollination overcoming pre-fertilization cross-incompatibility in Nicotiana.,Plant Biology

当前位置： X-MOL 学术 › Plant Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The morphological and physiological basis of delayed pollination overcoming pre-fertilization cross-incompatibility in Nicotiana.
Plant Biology ( IF 4.2 ) Pub Date : 2020-08-09 , DOI: 10.1111/plb.13168
S-Y Chen _{1,

2} , J-W Zhang _{1,

2} , X-M Wei _{1,

2} , K-L Tao _{1,

2} , Y-Z Niu ₃ , L Pan ₃ , Y-Y Zheng ₄ , W-G Ma ₄ , M-Q Wang ₅ , X-K Ou _{1,

2} , J-G Liao _{1,

2}

Affiliation

Delayed pollination is widely used to overcome pre‐fertilization incompatibility, but its regulatory mechanisms are unclear. When Nicotiana tabacum was cross‐pollinated with pollen of N. alata, the incompatibility occurring in the basal 1/4 region of the style (pollinated at anthesis: 0‐day‐old pistil) was overcome by delayed pollination (of 6‐day‐old pistil), and the morphological changes and corresponding physiological basis are explored here.
The structure and ultrastructure of the pistil were observed under fluorescence microscopy and transmission electron microscopy. Differentially expressed proteins were screened with a monoclonal antibody chip for Nicotiana, and protein expression and distribution were analysed by immunofluorescence. Cellulase and pectinase activities were tested using enzyme‐linked immunosorbent assay kits.
The style of Nicotiana is solid in the basal region and pollen tubes grow in the extracellular spaces (ECM) of the transmitting tissue (TTS) cells. Seven of the 22 identified proteins were cell wall‐associated proteins and were expressed at higher levels during pistil senescence. Cellulase and pectinase activities increased. The TTS cells in the basal 1/4 region of the 0‐day‐old style were polygonal and tightly arranged, with narrow ECM, but these were oval or partially dissolved in the 6‐day‐old pistil, leading to wider ECM and richer secretions.
The increased expression of cell wall proteins and enhanced enzyme activity during pistil senescence might partially be responsible for the cells becoming oval and the ECM enlarged, providing the morphological basis for delayed pollination overcoming the pre‐fertilization incompatibility between N. tabacum and N. alata.

中文翻译：

烟草延迟授粉克服受精前交叉不亲和性的形态和生理基础。

延迟授粉被广泛用于克服受精前不相容性，但其调节机制尚不清楚。当烟草与翼状烟草花粉异花授粉时，花柱基部 1/4 区域发生的不相容性（在花期授粉：0 日龄雌蕊）通过延迟授粉（6 天龄）得以克服。老雌蕊），并探讨其形态变化和相应的生理基础。
在荧光显微镜和透射电镜下观察雌蕊的结构和超微结构。利用烟草单克隆抗体芯片筛选差异表达蛋白，并通过免疫荧光分析蛋白表达和分布。使用酶联免疫吸附测定试剂盒测试纤维素酶和果胶酶活性。
烟草的花柱在基部区域是实心的，花粉管在传播组织（TTS）细胞的细胞外空间（ECM）中生长。 22 种已鉴定的蛋白质中有 7 种是细胞壁相关蛋白，并且在雌蕊衰老过程中表达水平较高。纤维素酶和果胶酶活性增加。 0日龄花柱基部1/4区域的TTS细胞呈多边形且排列紧密，ECM较窄，但在6日龄雌蕊中这些细胞呈椭圆形或部分溶解，导致ECM较宽且较丰富。分泌物。
雌蕊衰老过程中细胞壁蛋白表达的增加和酶活性的增强可能是细胞变成椭圆形和ECM增大的部分原因，为克服红花和翼花的受精前不亲和性的延迟授粉提供了形态学基础。

更新日期：2020-08-09

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11