Recombinant human bone morphogenetic protein 2 (rhBMP‐2) has been widely used in bone tissue engineering to enhance bone regeneration because of its osteogenic inductivity. However, clinical outcomes can vary depending on the scaffold materials used to deliver rhBMP‐2. In this study, 3D‐printed scaffolds with a ratio of 1:1 polycaprolactone and beta‐tricalcium phosphate (PCL/T50) were applied as carriers for rhBMP‐2 in mandibular bone defect models in dog models. Before in vivo application, in vitro experiments were conducted. Preosteoblast proliferation was not significantly different between scaffolds made of PCL/T50 and polycaprolactone alone (PCL/T0) regardless of rhBMP‐2 delivery. However, PCL/T50 showed an increased level of the alkaline phosphatase activity and mineralization assay when rhBMP‐2 was delivered. In in vivo, the newly formed bone volume of the PCL/T50 group was significantly increased compared with that of the PCL/T0 scaffolds regardless of rhBMP‐2 delivery. Histological examination showed that PCL/T50 with rhBMP‐2 produced significantly greater amounts of newly bone formation than PCL/T0 with rhBMP‐2. The quantities of scaffold remaining were lower in the PCL/T50 group than in the PCL/T0 group, although it was not significantly different. In conclusion, PCL/T50 scaffolds were advantageous for rhBMP‐2 delivery as well as for maintaining space for bone formation in mandibular bone defects.

中文翻译：

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三维打印的聚己内酯/β-磷酸三钙支架作为重组骨形成蛋白 2 的载体比单独的聚己内酯更有效

重组人骨形态发生蛋白 2 (rhBMP-2) 因其成骨诱导性而被广泛应用于骨组织工程以增强骨再生。然而，临床结果可能因用于递送 rhBMP-2 的支架材料而异。在这项研究中，聚己内酯和β-磷酸三钙 (PCL/T50) 比例为 1:1 的 3D 打印支架被应用于狗模型下颌骨缺损模型中的 rhBMP-2 载体。在体内应用之前，进行了体外实验。无论rhBMP-2的递送如何，前成骨细胞增殖在由PCL/T50和单独的聚己内酯（PCL/T0）制成的支架之间没有显着差异。然而，当给予 rhBMP-2 时，PCL/T50 显示碱性磷酸酶活性和矿化测定水平增加。在体内，与 PCL/T0 支架相比，PCL/T50 组新形成的骨量显着增加，而与 rhBMP-2 的输送无关。组织学检查表明，与含有 rhBMP-2 的 PCL/T0 相比，含有 rhBMP-2 的 PCL/T50 产生的新骨形成量明显更多。PCL/T50 组中剩余的支架数量低于 PCL/T0 组，但没有显着差异。总之，PCL/T50 支架有利于 rhBMP-2 的递送以及为下颌骨缺损中的骨形成保持空间。组织学检查表明，与含有 rhBMP-2 的 PCL/T0 相比，含有 rhBMP-2 的 PCL/T50 产生的新骨形成量明显更多。PCL/T50 组中剩余的支架数量低于 PCL/T0 组，但没有显着差异。总之，PCL/T50 支架有利于 rhBMP-2 的递送以及为下颌骨缺损中的骨形成保持空间。组织学检查表明，与含有 rhBMP-2 的 PCL/T0 相比，含有 rhBMP-2 的 PCL/T50 产生的新骨形成量明显更多。PCL/T50 组中剩余的支架数量低于 PCL/T0 组，但没有显着差异。总之，PCL/T50 支架有利于 rhBMP-2 的递送以及为下颌骨缺损中的骨形成保持空间。