The folic acid (FA) and doxorubicin (DOX) have been doped into the g‐C₃N₄/MoS₂ incorporated‐chitosan/ethyl cellulose (EC) core‐shell nanofibers for targeted delivery of FA and DOX against HeLa and MCF‐7 cell lines. The g‐C₃N₄/MoS₂ nanosheets and core‐shell nanofibers were characterized using Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, and UV–Vis tests. The drug loading factor, the degradation rate, and the DOX and FA release behavior from core‐shell nanofibers have been investigated. The pharmacokinetic results revealed the linear release with non‐Fickian diffusion of the both anticancer drugs from nanofibers during 7 days. The DAPI staining and MTT assays of the nanofibers immersed in MCF‐7 and HeLa cell lines were studied to determine the potential of DOX and FA doped‐core‐shell nanofibrous matrix for MCF‐7 and HeLa cells death in vitro. The maximum MCF‐7 and HeLa cells death percentages were found to be 89 and 85%, respectively, using EC/chitosan/g‐C₃N₄/MoS₂/DOX/FA core‐shell nanofibers after 7 days. The high activity of g‐C₃N₄/MoS₂/DOX/FA loaded‐core‐shell nanofibers for studied cancer cells killing was achieved.

中文翻译：

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从乙基纤维素/壳聚糖/g-C3N4/MoS2核壳纳米纤维中同时线性释放叶酸和阿霉素及其抗癌特性

叶酸 (FA) 和多柔比星 (DOX) 已掺杂到 g-C ₃ N ₄ /MoS ₂掺入壳聚糖/乙基纤维素 (EC) 核壳纳米纤维中，用于针对 HeLa 和 MCF 靶向递送 FA 和 DOX- 7 个细胞系。g-C ₃ N ₄ /MoS ₂使用傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜、透射电子显微镜和 UV-Vis 测试表征纳米片和核壳纳米纤维。已经研究了核壳纳米纤维的载药因子、降解速率以及 DOX 和 FA 释放行为。药代动力学结果显示两种抗癌药物在 7 天内从纳米纤维中线性释放和非 Fickian 扩散。研究了浸入 MCF-7 和 HeLa 细胞系中的纳米纤维的 DAPI 染色和 MTT 测定，以确定 DOX 和 FA 掺杂的核壳纳米纤维基质对 MCF-7 和 HeLa 细胞体外死亡的潜力。发现使用 EC/壳聚糖/g-C ₃ N的最大 MCF-7 和 HeLa 细胞死亡百分比分别为 89% 和 85%₄ /MoS ₂ /DOX/FA 核壳纳米纤维 7 天后。实现了 g-C ₃ N ₄ /MoS ₂ /DOX/FA 负载的核壳纳米纤维对癌细胞杀伤的高活性。