Descriptor ΔGC-O Enables the Quantitative Design of Spontaneously Blinking Rhodamines for Live-Cell Super-Resolution Imaging.,Angewandte Chemie International Edition

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Descriptor ΔGC-O Enables the Quantitative Design of Spontaneously Blinking Rhodamines for Live-Cell Super-Resolution Imaging.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-08-10 , DOI: 10.1002/anie.202010169
Weijie Chi _{1,

2} , Qinglong Qiao ₁ , Chao Wang _{1,

2} , Jiazhu Zheng ₁ , Wei Zhou ₁ , Ning Xu ₁ , Xia Wu ₂ , Xiao Jiang ₃ , Davin Tan ₂ , Zhaochao Xu ₁ , Xiaogang Liu ₂

Affiliation

Herein, we reported a simple, fast, and quantitative theoretical descriptor ΔG_C‐O that allows accurate predictions of a wide range of spontaneously blinking rhodamines. ΔG_C‐O denotes the Gibbs free energy differences between the closed and open forms of rhodamines and has a good linear relationship with experimental pK_cycl values. This correlation affords an effective guide for the quantitative designs of spontaneously blinking rhodamines and eliminates trial‐and‐error. We have validated the predictive power of ΔG_C‐O via the development of two spontaneously blinking rhodamines of different colors and enhanced brightness. We also demonstrated their super‐resolution imaging utilities in dynamic live‐cell imaging. We expect that ΔG_C‐O will greatly facilitate the efficient creations of spontaneously blinking fluorophores and aid the advancements of super‐resolution bioimaging techniques.

中文翻译：

描述符ΔGC-O可实现活细胞超分辨率成像自发闪烁若丹明的定量设计。

在本文中，我们报告了一种简单，快速且定量的理论描述符ΔG _C-O，可以准确预测各种自发闪烁的若丹明。Δ ģ _C-O表示罗丹明的关闭和打开形式之间的吉布斯自由能的差异，并具有良好的线性与实验p关系ķ _CYCL值。这种相关性为自发闪烁的若丹明的定量设计提供了有效的指导，并消除了反复试验的错误。我们已经验证了ΔG _C‐O的预测能力通过开发两种自发闪烁的罗丹明不同颜色和增强的亮度。我们还展示了他们在动态活细胞成像中的超分辨率成像实用程序。我们期望ΔG _C-O将极大地促进自发闪烁荧光团的高效产生，并有助于超分辨率生物成像技术的发展。

更新日期：2020-08-10

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