Abstract Controlling the supercooling state during ice crystallization process is important for differential scanning calorimetry (DSC) analysis. In cryopreservation solutions, the control of the nucleation temperature (Tn) is necessary as it allows the simulation of slow-freezing procedures. Herein, the effect of Snomax® on Tn value in aqueous solutions containing dimethyl-sulfoxide (Me2SO) (H2O + 10 %(v/v)Me2SO) was studied. The effect of Me2SO, a cryoprotectant, on nucleation control was investigated by evaluating λhet, which describes the influence of a molecule on the nucleation behaviour of an ice-nucleating agent (INA). Note that λhet is specific to the molecule added in solution and to the maximum melting temperature of the solution. The knowledge of λhet helps evaluate the ranges of the supercooling magnitude at which the INA in solution can trigger nucleation. This study shows how Snomax can be used in DSC experiments using the H2O + 10 %(v/v)Me2SO solution when ice nucleation must be controlled.

中文翻译：

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在含二甲基亚砜的水溶液中使用 Snomax® 进行冰诱导，用于 DSC 实验

摘要 控制冰结晶过程中的过冷状态对于差示扫描量热法 (DSC) 分析很重要。在冷冻保存解决方案中，控制成核温度 (Tn) 是必要的，因为它允许模拟慢速冷冻程序。在此，研究了 Snomax® 对含有二甲基亚砜 (Me2SO) (H2O + 10%(v/v)Me2SO) 的水溶液中 Tn 值的影响。通过评估 λhet 来研究 Me2SO（一种冷冻保护剂）对成核控制的影响，λhet 描述了分子对冰成核剂 (INA) 成核行为的影响。请注意，λhet 特定于添加到溶液中的分子和溶液的最高熔化温度。λhet 的知识有助于评估溶液中的 INA 可以触发成核的过冷幅度范围。本研究展示了如何在必须控制冰成核时使用 H2O + 10%(v/v)Me2SO 溶液的 DSC 实验中使用 Snomax。