Abstract We report the preparation of microporous surfaces prepared from immiscible polymer blends using the Breath Figures methodology. The selected polymer blend is formed by two biodegradable polymers i.e. poly(e-caprolactone) and poly(lactic acid) both known to be biocompatible and presenting two distinct kinetics of biodegradation. These two polymers form immiscible polymer blends upon solvent casting in low relative humidity conditions. However, when quenching the melt using fast cooling processes or by evaporation in a moist atmosphere, micrometer size phase-separated domains have been clearly identified by Raman Confocal. This allowed us to analyze cell adhesion as well as proliferation on both planar (those obtained from melt quenching) and microporous surfaces (those obtained by the breath figures approach) formed from different blend compositions. Using C166-GFP and RAW264.7 cell types as a model we could observe an improved adhesion of the C166-GFP on top of the microporous surfaces evidencing the role of the surface topography on the cell adhesion process. In addition, preliminary results on the RAW264.7 cell adhesion indicated the polarization to M2 phenotypes instead of M1 which is a pre-requisite for the potential use of this material for implantology purposes.

中文翻译：

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从不混溶的聚合物共混物制备多孔膜：表面结构对细胞粘附的作用

摘要我们报告了使用呼吸图方法由不混溶的聚合物共混物制备的微孔表面的制备。选定的聚合物共混物由两种可生物降解的聚合物形成，即聚（ε-己内酯）和聚（乳酸），这两种聚合物均已知具有生物相容性并具有两种不同的生物降解动力学。这两种聚合物在低相对湿度条件下通过溶剂浇铸形成不混溶的聚合物共混物。然而，当使用快速冷却过程或通过在潮湿气氛中蒸发来淬火熔体时，拉曼共聚焦已清楚地识别出微米尺寸的相分离域。这使我们能够分析由不同共混组合物形成的平面（从熔体淬火获得的那些）和微孔表面（通过呼吸图方法获得的那些）上的细胞粘附和增殖。使用 C166-GFP 和 RAW264.7 细胞类型作为模型，我们可以观察到 C166-GFP 在微孔表面上的粘附改善，证明了表面形貌对细胞粘附过程的作用。此外，RAW264.7 细胞粘附的初步结果表明，极化到 M2 表型而不是 M1，这是该材料可能用于种植学目的的先决条件。7 种细胞类型作为模型，我们可以观察到 C166-GFP 在微孔表面上的粘附改善，证明了表面形貌对细胞粘附过程的作用。此外，RAW264.7 细胞粘附的初步结果表明，极化到 M2 表型而不是 M1，这是该材料可能用于种植学目的的先决条件。7 种细胞类型作为模型，我们可以观察到 C166-GFP 在微孔表面上的粘附改善，证明了表面形貌对细胞粘附过程的作用。此外，RAW264.7 细胞粘附的初步结果表明，极化到 M2 表型而不是 M1，这是该材料可能用于种植学目的的先决条件。