Chitosan exhibiting excellent functional properties was found to be potential in a wide variety of industrial and biomedical applications. The lack of biological activities and the poor mechanical properties somewhat limit its applications. To address these limitations, chemical modification has been proved to be an efficient method. Here, we immobilized arginine-glycine-aspartic acid (RGD) on chitosan molecules and incorporated the chitosan with Poly(L-lactide-co-glycolide)-Poly(ethylene glycol) (PLGA-PEG) and β-tricalcium phosphate (β-TCP) nanoparticles to obtain a chitosan-based scaffold with enhanced mechanical and biocompatible performance. XPS and FT-IR confirmed the successful grafting of RGD peptides onto the chitosan chain. The mechanical properties, hydrophilicity and degradation behaviors of the membrane were characterized by stress-strain, water contact angle, and weight loss, respectively. Cytotoxicity, cellular adhesion, and cellular viability of the scaffolds were studied by MTT assay, cell adhesion, and cell survival experiments, respectively. The advantageous mechanical properties and the hydrophilic surface of the chitosan-based scaffold were achieved by blending with PLGA-PEG. The preliminary biocompatibility studies revealed that all the scaffolds were cell compatible, and the hydrophilic surface was more suitable for cell adhesion. The scaffolds grafted with RGD exhibited high cell adhesion rate than the pure chitosan. The results suggested that the surface hydrophilicity and biocompatibility of the chitosan-based scaffolds play an important role in enhancing cell adhesion and growth.

发现具有优异功能特性的壳聚糖在多种工业和生物医学应用中具有潜力。缺乏生物活性和较差的机械性能在一定程度上限制了其应用。为了解决这些限制，化学修饰已被证明是一种有效的方法。在这里，我们将精氨酸-甘氨酸-天冬氨酸（RGD）固定在壳聚糖分子上，并将壳聚糖与聚（L-丙交酯-共-乙交酯）-聚（乙二醇）（PLGA-PEG）和β-磷酸三钙（β- TCP）纳米颗粒，以获得具有增强的机械和生物相容性性能的壳聚糖基支架。XPS和FT-IR证实RGD肽已成功嫁接到壳聚糖链上。机械性能 膜的亲水性和降解行为分别由应力应变，水接触角和重量损失表征。通过MTT测定，细胞粘附和细胞存活实验分别研究了支架的细胞毒性，细胞粘附和细胞生存力。壳聚糖基支架的有利机械性能和亲水性表面是通过与PLGA-PEG共混获得的。初步的生物相容性研究表明，所有支架均与细胞相容，并且亲水性表面更适合细胞粘附。移植RGD的支架比纯壳聚糖具有更高的细胞粘附率。