The aim of this study was to evaluate whether fingerprints are suitable to be applied as the biometric identification samples by testing the orally administered drugs needs to be taken daily. The dosage of BETALOC® was administered to subjects following single and multiple doses and its active ingredient metoprolol and its main metabolite α-hydroxyl metoprolol were selected as the analytes. The subjects washed their hands and pressed fingertips onto glass slides at fixed sampling points (from 1 h to 7 days), and the analytes were extracted using cotton swabs 30 times followed by ultrasonic assistance in 30℃ methanol solution for 5 min with working power of 2000 W after optimization. The drugs in blood were taken from their elbow vein and deproteinized before analysis. Analysis were performed using liquid chromatography–tandem mass spectrometry (LC/MS/MS), and their concentration time course in fingerprints and blood were evaluated and compared. Results showed that metoprolol was detected 1 h after ingestion both in fingerprints and blood, while α-hydroxyl metoprolol was detected from sampling points of 2 h in fingerprint and 3 h in blood, respectively. Drugs could be detected for longer periods in blood than in fingerprint in single dose administration. However, in multiple doses, they could reach a steady detectable state in fingerprints from the fifth day after oral administration, and could serve as a more rapid and simpler alternative for drug analysis. We demonstrate that fingerprints could be applied as the biometric identification samples for orally administered drugs in multiple-doses, and could be applied to drug testing in criminal investigations.

这项研究的目的是通过测试需要每天口服的药物来评估指纹是否适合用作生物特征识别样品。单次和多次给药后向受试者给药，并选择其活性成分美托洛尔及其主要代谢物α-羟基美托洛尔作为分析物。受试者在固定的采样点（从1小时到7天）洗手并用指尖按在载玻片上，用棉签提取分析物30次，然后在30℃甲醇溶液中超声辅助5分钟，工作能力为优化后为2000W。血液中的药物是从肘部静脉取出并进行脱蛋白处理后再进行分析的。使用液相色谱-串联质谱（LC / MS / MS）进行分析，并评估和比较它们在指纹和血液中的浓缩时间。结果显示，摄入1 h后，指纹和血液中均检测到美托洛尔，而从指纹2 h和血液3 h的采样点中检测到α-羟基美托洛尔。与单剂给药相比，在血液中检测到的药物比在指纹中检测的时间更长。但是，从口服后的第五天开始，在多剂量下，它们可能会在指纹中达到稳定的可检测状态，并且可以用作药物分析的更快速，更简单的替代方法。我们证明了指纹可以作为多剂量口服药物的生物特征识别样品，