Background

Age-associated decline in nicotinamide adenine dinucleotide (NAD) tissue levels has emerged as potential driving mechanism in the establishment of energy metabolism perturbations in the context of chronic diseases, notably heart failure.

Objective

The aim of this study was to measure the blood NAD levels in a healthy blood donor population and in a population of elderly patients hospitalized for decompensated heart failure.

Method

Whole blood sample was collected from 151 healthy voluntary blood donors, aged 19 to 68 years, and from 19 patients aged 75 to 101 years and hospitalized for decompensated heart failure in a geriatric ward. Metabolites were extracted by the hot buffered ethanol procedure and NAD was quantified in triplicate for each sample.

Results

The mean concentration of NAD in blood of healthy donors was 23.4 (SD 4.05) μmol/L. There was no significant correlation between NAD levels and donors' age nor sex in the healthy population when studied as a whole. However, the linear regression curves of NAD concentration plotted against age differed between males and females (p = 0.0283) with a trend in males to decline with age that was not observed in females. The mean concentration of NAD in whole blood samples of the geriatric population was 20.7 (SD 3.6) μmol/L (p = 0.007 versus the healthy blood donor population). There were no differences between males and females (p = 0.7) nor between patients with ejection fraction inferior or superior to 50% (p = 0.86) in the geriatric population.

Conclusion

This study highlighted a diminution of NAD blood levels for elderly patients hospitalized for decompensated heart failure in comparison to a healthy population, suggesting that new therapeutics to restore NAD stock and energy metabolism would be a major progress in the management of this type of geriatric patients.

中文翻译：

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在因心力衰竭住院的非常老的患者中，血液NAD水平降低。

背景

烟酰胺腺嘌呤二核苷酸（NAD）组织水平与年龄相关的下降已成为在慢性疾病（尤其是心力衰竭）背景下建立能量代谢紊乱的潜在驱动机制。

目的

这项研究的目的是测量健康献血者人群和因失代偿性心力衰竭住院的老年患者的血液NAD水平。

方法

从151名年龄在19至68岁的健康自愿献血者和19名年龄在75至101岁的患者中采集全血样本，并在老年病房接受因代偿性心力衰竭住院。通过热缓冲乙醇程序提取代谢物，并对每个样品进行三次重复的NAD定量。

结果

健康献血者血液中NAD的平均浓度为23.4（SD 4.05）μmol/ L。整体研究时，NAD水平与健康人群中供者的年龄或性别之间无显着相关性。但是，男性和女性之间相对于年龄绘制的NAD浓度线性回归曲线有所不同（p  = 0.0283），男性随着年龄的增长而下降的趋势在女性中没有观察到。老年人群全血样品中NAD的平均浓度为20.7（SD 3.6）μmol/ L（ 相对于健康献血者人群，p = 0.007）。男女之间无差异（p  = 0.7），射血分数低于或高于50％（p = 0.86）。

结论

这项研究强调，与健康人群相比，因失代偿性心力衰竭住院的老年患者的NAD血药浓度降低，这表明恢复NAD储备和能量代谢的新疗法将是这类老年患者管理的重大进展。