This study aims to explore the mechanism of fluid shear stress in regulating the primary cilia assembly or disassembly in human umbilical vein endothelial cells (hUVECs) using microfluidic chamber experiments. Immunofluorescence analysis showed that primary cilia assembled under disturbed fluid shear stress (DF) of 1 dyne/cm², while disassembled under unidirectional shear stress (USS) of 15 dynes/cm². Disheveled (Dvl2) in Wnt signaling pathway was effectively co-immunoprecipitated with Bardet–Biedl syndrome proteins 8 (Bbs8) and γ-tubulin. Compared with those in the control group, the percentages of ciliated cells with Dvl2 overexpression were found to be 67% and 59.667%, respectively, under USS and DF (an increment of 21–38.7%); while, those with Dvl2 silencing were 16% and 32.667%, respectively, under USS and DF (a decrement of 23–30%). Further, the expression of Bbs8 and γ-tubulin was decreased by RNA interference of Dvl2 but increased with Dvl2 overexpression. The results indicated that Dvl2 played a pivotal role during DF-induced primary cilia assembly, and was important for apical docking of basal bodies through Bbs8 and γ-tubulin.

这项研究旨在探讨微剪切室实验中流体剪切应力在调节人脐静脉内皮细胞（hUVECs）初级纤毛组装或拆卸中的机制。免疫荧光分析表明，初级纤毛在1达因/厘米^2的扰动流体剪切应力（DF）下组装，而在15达因/ cm ^2的单向剪切应力（USS）下分解。。Wnt信号通路中的紊乱（Dvl2）与Bardet-Biedl综合征蛋白8（Bbs8）和γ-微管蛋白有效地共免疫沉淀。与对照组相比，在USS和DF条件下，Dvl2过表达的纤毛细胞百分比分别为67％和59.667％（增加21–38.7％）；而在USS和DF下，具有Dvl2沉默的人分别为16％和32.667％（减少了23–30％）。此外，Dvl2的RNA干扰使Bbs8和γ-微管蛋白的表达降低，但随着Dvl2的过表达而增加。结果表明，Dvl2在DF诱导的初级纤毛组装过程中起着关键作用，对于通过Bbs8和γ-微管蛋白对基体的顶部对接非常重要。