Graphene oxide (GO)-based dispersive solid phase extraction (D-SPE) method combined with multi-step preparation has been put forward for the evaluation of trace ochratoxin A (OTA) in chicken liver. The homogenized tissue sample was initially clean-up and extracted with petroleum ether, ethyl acetate and 0.1 mol/L sodium bicarbonate solution and then adsorbed using GO originally targeting for OTA. The adsorbed OTA was then eluted twice by 1 mL of methanol and finally quantified by high performance liquid chromatography fluorescence detection (HPLC-FD). As a result, the matrix effect of chicken liver tissue was removed to a great extent after a series of processing steps. Under the optimum conditions, the functionality of the method was attested and this method demonstrated proper sensitivity, with detection and quantification limits equal to 0.02 ng/mL and 0.04 ng/mL, respectively. The method turned out to be accurate with recoveries ranging from 86.4 to 102.9%. Furthermore, the established method was applied to assess OTA contamination in different batches chicken liver, which revealed that two supermarket-sold and three individual domestic samples were contaminated with OTA (33% frequency) and the proposed method was suitable for continuous monitoring for OTA in chicken liver to ensure food safety.

中文翻译：

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基于氧化石墨烯吸附剂的分散固相萃取结合多重预处理净化分析鸡肝中痕量赭曲霉毒素 A

提出了基于氧化石墨烯 (GO) 的分散固相萃取 (D-SPE) 方法结合多步制备方法来评价鸡肝中痕量赭曲霉毒素 A (OTA)。匀浆后的组织样品首先用石油醚、乙酸乙酯和 0.1 mol/L 碳酸氢钠溶液进行净化和提取，然后使用最初针对 OTA 的 GO 进行吸附。然后将吸附的 OTA 用 1 mL 甲醇洗脱两次，最后通过高效液相色谱荧光检测 (HPLC-FD) 进行定量。结果，经过一系列处理步骤后，鸡肝组织的基质效应在很大程度上被去除。在最佳条件下，证明了该方法的功能性，并且该方法显示出适当的灵敏度，检测限和定量限等于 0。分别为 02 ng/mL 和 0.04 ng/mL。结果证明该方法是准确的，回收率为 86.4% 至 102.9%。此外，将建立的方法应用于评估不同批次鸡肝中的OTA污染，结果表明两个超市销售的样品和三个单独的国内样品被OTA污染（频率为33％），并且该方法适用于对OTA的连续监测。鸡肝确保食品安全。