In humans, sexual dimorphism can manifest in many ways and it is widely studied in several knowledge fields. It is increasing the evidence that also cells differ according to sex, a correlation still little studied and poorly considered when cells are used in scientific research. Specifically, our interest is on the sex-related dimorphism on the human mesenchymal stem cells (hMSCs) transcriptome. A systematic meta-analysis of hMSC microarrays was performed by using the Transcriptome Mapper (TRAM) software. This bioinformatic tool was used to integrate and normalize datasets from multiple sources and allowed us to highlight chromosomal segments and genes differently expressed in hMSCs derived from adipose tissue (hADSCs) of male and female donors. Chromosomal segments and differentially expressed genes in male and female hADSCs resulted to be related to several processes as inflammation, adipogenic and neurogenic differentiation and cell communication. Obtained results lead us to hypothesize that the donor sex of hADSCs is a variable influencing a wide range of stem cell biologic processes. We believe that it should be considered in biologic research and stem cell therapy.

中文翻译：

---

人类脂肪间充质干细胞的性别特异性转录组差异

在人类中，性别二态性可以通过多种方式表现出来，并且在多个知识领域得到广泛研究。越来越多的证据表明细胞也因性别而异，当细胞用于科学研究时，这种相关性仍然很少被研究和考虑。具体而言，我们的兴趣在于人类间充质干细胞 (hMSCs) 转录组的性别相关二态性。使用转录组映射器 (TRAM) 软件对 hMSC 微阵列进行了系统的荟萃分析。这种生物信息学工具用于整合和标准化来自多个来源的数据集，并允许我们突出显示在源自男性和女性供体的脂肪组织 (hADSC) 的 hMSC 中不同表达的染色体片段和基因。雄性和雌性 hADSCs 中的染色体片段和差异表达基因与炎症、成脂和神经源性分化以及细胞通讯等几个过程有关。获得的结果使我们假设 hADSCs 的供体性别是一个影响广泛干细胞生物学过程的变量。我们认为在生物研究和干细胞治疗中应该考虑它。