The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches based on liquid chromatography−electrospray ionization−tandem mass spectrometry (LC−ESI−MS/MS) for investigating the protein profile of three pasta samples containing different percentages of narrow-leaf lupin flour were carried out. The untargeted method permitted the identification of the main acidic globulins (α-conglutin, β-conglutin, and δ-conglutin) and the comparison of their profile with raw lupin flour. The targeted method, based on High-performance liquid chromatography electrospray ionization tandem mass spectrometry HPLC-Chip-Multiple Reaction Monitoring (MRM) mode, allowed the quantification of γ-conglutin, the main hypoglycemic component of lupin protein: its concentration was around 2.25 mg/g in sample A, 2.16 mg/g in sample D, and 0.57 mg/g in sample F.

中文翻译：

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非靶向和靶向质谱法分析富羽扇豆面食中的窄叶羽扇豆蛋白。

已经证明，用豆类谷物特别是羽扇豆补充不同的食品可以提供有益的健康益处，特别是在预防心血管疾病方面。在这项工作中，基于液相色谱-电喷雾电离串联质谱（LC-ESI-MS / MS）的无标签定量无靶和无靶方法用于研究三种包含不同百分比的窄叶羽扇豆粉的意大利面样品的蛋白质谱执行。无目标方法可以鉴定主要的酸性球蛋白（α-凝集素，β-凝集素和δ-凝集素），并与生羽扇豆粉进行比较。有针对性的方法，