NOGO-A immunolabeling is present in glial cells and some neurons of the recovering lumbar spinal cord in lizards,Journal of Morphology

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NOGO-A immunolabeling is present in glial cells and some neurons of the recovering lumbar spinal cord in lizards
Journal of Morphology ( IF 1.5 ) Pub Date : 2020-08-08 , DOI: 10.1002/jmor.21245
Lorenzo Alibardi ₁

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The transected lumbar spinal cord of lizards was studied for its ability to recover after paralysis. At 34 days post‐lesion about 50% of lizards were capable of walking with a limited coordination, likely due to the regeneration of few connecting axons crossing the transection site of the spinal cord. This region, indicated as “bridge”, contains glial cells among which oligodendrocytes and their elongation that are immunolabeled for NOGO‐A. A main reactive protein band occurs at 100–110 kDa but a weaker band is also observed around 240 kDa, suggesting fragmentation of the native protein due to extraction or to physiological processing of the original protein. Most of the cytoplasmic immunolabeling observed in oligodendrocytes is associated with vesicles of the endoplasmic reticulum. Also, the nucleus is labeled in some oligodendrocytes that are myelinating sparse axons observed within the bridge at 22–34 days post‐transection. This suggests that axonal regeneration is present within the bridge region. Immunolabeling for NOGO‐A shows that the protein is also present in numerous reactive neurons, in particular motor‐neurons localized in the proximal stump of the transected spinal cord. Ultrastructural immunolocalization suggests that NOGO is synthesized in the ribosomes of these neurons and becomes associated with the cisternae of the endoplasmic reticulum, probably following a secretory pathway addressed toward the axon. The present observations suggest that, like for the regenerating spinal cord of fish and amphibians, also in lizard NOGO‐A is present in reactive neurons and appears associated to axonal regeneration and myelination.

中文翻译：

NOGO-A 免疫标记存在于蜥蜴恢复中的腰脊髓的神经胶质细胞和一些神经元中

研究了蜥蜴横断的腰椎脊髓在瘫痪后的恢复能力。在损伤后 34 天，大约 50% 的蜥蜴能够以有限的协调性行走，这可能是由于少数连接轴突穿过脊髓横断部位的再生。这个区域，表示为“桥”，包含神经胶质细胞，其中少突胶质细胞及其伸长被免疫标记为 NOGO-A。主要的反应蛋白条带出现在 100–110 kDa 处，但在 240 kDa 附近也观察到一条较弱的条带，这表明由于提取或原始蛋白质的生理加工，天然蛋白质发生了断裂。在少突胶质细胞中观察到的大多数细胞质免疫标记与内质网的囊泡有关。还，细胞核被标记在一些少突胶质细胞中，这些少突胶质细胞是在横断后 22-34 天在桥内观察到的有髓鞘的稀疏轴突。这表明桥区域内存在轴突再生。NOGO-A 的免疫标记表明，该蛋白质也存在于许多反应性神经元中，特别是位于横断脊髓近端残端的运动神经元。超微结构免疫定位表明 NOGO 是在这些神经元的核糖体中合成的，并与内质网的池相关联，可能遵循一条针对轴突的分泌途径。目前的观察表明，就像鱼类和两栖动物的再生脊髓一样，

更新日期：2020-08-08

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