This study was to analyses the miRNAs role in cervical cancer and possibilities of microRNA-based markers as diagnostic tools. Genome wide analysis was performed for CNV detection using PennCNV and QuantiSNP. The associated mRNA qRT-PCR detection was used to measure quantities of microRNA gene expression. More than 10 CNV regions has a significant relationship with cervical cancer risk for both CNV detection algorithms. A total of 34 CNVs was detected by QuantiSNP while it was 27 in case of PennCNV, among which 22 CNVs was found to be overlapping between these two algorithms. the mRNA was analyzed for its expression on 36 carvical tumor normal tissue pairs of four targets i.e., MAP3K3, RIPK2, DIRAS3 and GAS7. These infers that there was a significant downregulation of all the four genes cervical tumor. Our results showed that miR-182 can modulate the expression of FAM83H, DIRAS3, RIPK2 and MAP3K3 in cervical cancer. Therefore, indicated that miR-182 can acts through these signaling pathway in proliferation of cervical cancer cells. The expression of tumor modulator miRNAs can be controlled by miRNA replacement therapy. Several miRNAs have been used for this purpose. The modulation of various signaling pathway and proteins in cervical cancer cells by miR-182 needs further clarification.

本研究旨在分析 miRNA 在宫颈癌中的作用以及基于 microRNA 的标记物作为诊断工具的可能性。使用 PennCNV 和 QuantiSNP 对 CNV 检测进行全基因组分析。相关的 mRNA qRT-PCR 检测用于测量 microRNA 基因表达的数量。对于两种 CNV 检测算法，超过 10 个 CNV 区域与宫颈癌风险有显着关系。QuantiSNP共检测到34个CNV，而PennCNV检测到27个，其中22个CNV被发现在这两种算法之间重叠。分析 mRNA 在 36 个颈癌正常组织对的四个靶标（即MAP3K3、RIPK2、DIRAS3和GAS7 ）上的表达. 这些推断宫颈肿瘤的所有四个基因均显着下调。我们的研究结果表明，miR-182 可以调节宫颈癌中 FAM83H、DIRAS3、RIPK2 和 MAP3K3 的表达。因此，表明miR-182可以通过这些信号通路在宫颈癌细胞增殖中发挥作用。肿瘤调节剂 miRNA 的表达可以通过 miRNA 替代疗法来控制。为此目的使用了几种 miRNA。miR-182对宫颈癌细胞中各种信号通路和蛋白质的调节需要进一步阐明。