The objective of this study was to characterize the biofilms formed by Salmonella enterica serotype Agona, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) after 12, 48, 72, 120 and 240 h of incubation at 10 °C. Biofilms containing a single species, together with dual-species biofilms in which S. enterica and a Gram-positive bacterium existed in combination, were formed on polystyrene and evaluated by using confocal laser scanning microscopy (CLSM). All strains were able to form biofilm. The greatest biovolume in the observation field of 14,161 μm² was observed for mono-species biofilms after 72 h, where biovolumes of 94,409.0 μm³ ± 2131.0 μm³ (S. enterica), 58,418.3 μm³ ± 5944.9 μm³ (L. monocytogenes), 68,020.8 μm³ ± 5812.3 μm³ (MRSA) and 59,280.0 μm³ ± 4032.9 μm³ (VRE) were obtained. In comparison with single-species biofilms, the biovolume of S. enterica was higher in the presence of MRSA or VRE after 48, 72 and 120 h. In dual-species biofilms, the bacteria showed a double-layer distribution pattern, with S. enterica in the top layer and Gram-positive bacteria in the bottom layer. This spatial disposition should be taken into account when effective strategies to eliminate biofilms are being developed.

这项研究的目的是表征在孵育12、48、72、120和240小时后，由肠炎沙门氏菌血清型Agona，单核细胞增生李斯特菌，耐甲氧西林金黄色葡萄球菌（MRSA）和耐万古霉素肠球菌（VRE）形成的生物膜。在10°C。含有单一物种，与其中双物种生物膜一起生物膜肠道沙门氏菌和革兰氏阳性细菌组合存在，形成于聚苯乙烯，并通过使用共聚焦激光扫描显微镜（CLSM）来评价。所有菌株都能形成生物膜。在14161微米的观察视场的最大biovolume ²观察到单物种的生物膜72小时，之后其中的94,409.0微米biovolumes ³  ±2131.0微米³（沙门氏菌），58,418.3微米³  ±5944.9微米³（李斯特菌），68,020.8微米³  ±5812.3微米³（MRSA）和59,280.0微米³  ±4032.9微米³得到（VRE）。与单物种生物膜相比，在48、72和120小时后，在存在MRSA或VRE的情况下，肠炎链球菌的生物量更高。在双物种生物膜中，细菌表现出双层分布模式，肠球菌在顶层，革兰氏阳性细菌在底层。在制定消除生物膜的有效策略时，应考虑这种空间布置。