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Transcriptome analysis of the Larimichthys polyactis under heat and cold stress
Cryobiology ( IF 2.3 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.cryobiol.2020.06.014
Tianqi Chu 1 , Feng Liu 2 , Gaochan Qin 1 , Wei Zhan 2 , Mengjie Wang 1 , Bao Lou 2
Affiliation  

The small yellow croaker (Larimichthys polyactis) is an economically important marine fish that is widely distributed in the East Sea, Yellow Sea and Bohai of China. However, the wild populations of L. polyactis are severely depleted, and there is currently a developing large-scale artificial propagation of this fish for aquaculture. However, the current variety of L. polyactis that is cultivated is not capable to coping with large fluctuations in temperature. Therefore, it is important to understand the molecular mechanisms that are activated in response to temperature stress in the L. polyactis. Here, we conducted transcriptomic analysis of the liver of L. polyactis under heat and cold stress. A total of 270,844,888, 265,727,006 and 259,666,218 clean reads were generated from high temperature group, low temperature group and control group, respectively, and comparing expression of genes in these transcriptomes, 10,878 unigenes that were differential expressed were identified. Seventeen of the differentially expressed unigenes were validated by qRT-PCR. Pathway enrichment analysis identified that the ER pathway, immune signaling pathway and metabolic response pathway were affected by temperature stress. The results of this study provide a comprehensive overview of temperature stress-induced transcriptional patterns in liver tissues of the L. polyactis. In addition, these results can guide future molecular studies of heat and cold stress response in this species for improving the stock used for aquaculture.
更新日期:2020-10-01
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