Application of nanomaterials is becoming the most effective strategy of elicitation to produce a desirable level of plant biomass with complex medicinal compounds. This study was, designed to check the influence of commercial iron nanoparticles (FeNPs) on physical growth characteristics, antioxidant status and production of steviol glycosides of in vitro grown Stevia rebaudiana. Results indicated that lower concentrations of FeNPs (45 µg/L) had a positive influence on morphological growth parameters. At a higher dose (90, and 135 µg/L) FeNPs in culture media were found detrimental to growth characteristics and development. Furthermore, the stress caused by FeNPs at 135 µg/L in cultures produced higher levels of total phenolic content (3.2 ± 0.042 mg/g dry weight: DW), total flavonoid content (1.6 ± 0.022 mg/g DW and antioxidant activity (73 ± 4.6%). In addition, plants grown in the presence of FeNPs at 90 µg/L resulted in higher enzymatic antioxidant activities (SOD = 3.2 ± 0.042 U/mg; POD = 2.1 ± 0.026 U/mg; CAT = 2.6 ± 0.034 U/mg and APx = 3.3 ± 0.043 U/mg), respectively. Furthermore, exposure to a low dose of FeNPs (45 µg/L) exhibited the maximum amount of stevioside (stevioside: 4.2 ± 0.058 mg/g (DW) and rebaudioside A: 4.9 ± 0.068 mg/g DW) as compared to high doses. The current investigation confirms the effectiveness of FeNPs in growth media and offers a suitable prospect for commercially desirable production of S. rebaudiana biomass with higher sweet glycosides profiles in vitro.

纳米材料的应用正成为诱导产生具有理想药用水平的植物生物质的最有效的策略。这项研究旨在检查商用铁纳米颗粒（FeNPs）对体外生长的甜叶菊甜叶菊的物理生长特征，抗氧化剂状态和甜菊醇糖苷生产的影响。结果表明，较低浓度的FeNPs（45 µg / L）对形态生长参数具有积极影响。发现在较高剂量（90和135 µg / L）的培养基中，FeNPs不利于生长特性和发育。此外，FeNPs在135 µg / L的培养物中引起的胁迫产生更高水平的总酚含量（3.2±0.042 mg / g干重：DW），总黄酮含量（1.6±0.022 mg / g DW和抗氧化活性（73） ±4.6％）。此外，以90 µg / L的FeNPs存在的植物可产生更高的酶促抗氧化活性（SOD = 3.2±0.042 U / mg； POD = 2.1±0.026 U / mg； CAT = 2.6±0.034 U / mg和APx分别为3.3±0.043 U / mg），此外，暴露于低剂量的FeNPs（45 µg / L）时，甜菊糖甙含量最高（甜菊糖甙为4.2±0）。058毫克/克（DW）和莱鲍迪甙A：4.9±0.068毫克/克DW）。目前的研究证实了FeNP在生长培养基中的有效性，并为商业上理想的生产生产提供了合适的前景。S. rebaudiana生物量具有较高的甜甙体外分布。