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Development and validation of a specific-stability indicating liquid chromatography method for quantitative analysis of pterostilbene: application in food and pharmaceutical products.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-08-07 , DOI: 10.1039/d0ay00989j Mairique Waszczuk 1 , Sara Elis Bianchi , Simony Martiny , Vanessa Pittol , Denise S Lacerda , Alex Sander da Rosa Araújo , Valquiria Linck Bassani
Analytical Methods ( IF 2.7 ) Pub Date : 2020-08-07 , DOI: 10.1039/d0ay00989j Mairique Waszczuk 1 , Sara Elis Bianchi , Simony Martiny , Vanessa Pittol , Denise S Lacerda , Alex Sander da Rosa Araújo , Valquiria Linck Bassani
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Pterostilbene is a natural constituent with numerous preventive and therapeutic properties used for treating a wide range of human diseases. It has been isolated from blueberries in high concentrations. A versatile specific-stability indicating liquid chromatography method for reliable quantitative determination of pterostilbene in blueberry extracts was developed. In the stability-indicating analysis, pterostilbene samples were exposed to stress conditions: temperature, UV light, and oxidative, acid and alkaline media. The robustness of the method was evaluated using a Box–Behnken experimental design. Pterostilbene was stable in acid and alkaline media, but unstable when exposed to oxidation, high temperature, and UV light. Using the proposed method, it was demonstrated that degradation products did not interfere in the analyte analysis. The method revealed to be simple, fast, selective, linear, precise and accurate for the quantitative analysis of pterostilbene in Vaccinium myrtillus extract. Besides being a specific-stability indicating method, it demonstrated to be applicable to quantify pterostilbene in pterostilbene:β-cyclodextrin complexes and pterostilbene:β-cyclodextrin:hydrophilic polymer ternary systems. These findings are relevant for the development of food, pharmaceutical or cosmetic products containing pterostilbene or blueberry extracts.
中文翻译：
开发和验证一种用于定量鉴定蝶形蝶烯的比色稳定性液相色谱方法：在食品和药品中的应用。
蝶烯是一种天然成分，具有多种预防和治疗特性，可用于治疗多种人类疾病。它是从蓝莓中高浓度分离出来的。开发了一种通用的比稳定性指示液相色谱方法，用于可靠地定量测定蓝莓提取物中的紫檀萜。在稳定性指示分析中，将蝶形til样品暴露于应力条件下：温度，紫外线，以及氧化，酸性和碱性介质。使用Box–Behnken实验设计评估了该方法的鲁棒性。蝶烯在酸性和碱性介质中稳定，但在暴露于氧化，高温和紫外线下则不稳定。使用所提出的方法，证明了降解产物不会干扰分析物的分析。牛痘菌提取物。除了作为一种特定的稳定性指示方法外，它还适用于定量蝶草烯：β-环糊精复合物和蝶草烯：β-环糊精：亲水性聚合物三元体系中的蝶呤。这些发现与开发含有紫檀皮或蓝莓提取物的食品，药品或化妆品有关。
更新日期：2020-09-17
中文翻译：
开发和验证一种用于定量鉴定蝶形蝶烯的比色稳定性液相色谱方法：在食品和药品中的应用。
蝶烯是一种天然成分，具有多种预防和治疗特性，可用于治疗多种人类疾病。它是从蓝莓中高浓度分离出来的。开发了一种通用的比稳定性指示液相色谱方法，用于可靠地定量测定蓝莓提取物中的紫檀萜。在稳定性指示分析中，将蝶形til样品暴露于应力条件下：温度，紫外线，以及氧化，酸性和碱性介质。使用Box–Behnken实验设计评估了该方法的鲁棒性。蝶烯在酸性和碱性介质中稳定，但在暴露于氧化，高温和紫外线下则不稳定。使用所提出的方法，证明了降解产物不会干扰分析物的分析。牛痘菌提取物。除了作为一种特定的稳定性指示方法外，它还适用于定量蝶草烯：β-环糊精复合物和蝶草烯：β-环糊精：亲水性聚合物三元体系中的蝶呤。这些发现与开发含有紫檀皮或蓝莓提取物的食品，药品或化妆品有关。
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