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Tailoring Pseudo-Zwitterionic Bifunctionalized Silica Nanoparticles: From Colloidal Stability to Biological Interactions.
Langmuir ( IF 3.7 ) Pub Date : 2020-08-07 , DOI: 10.1021/acs.langmuir.0c01545 Francine Ramos Scheffer 1, 2 , Camila Pedroso Silveira 1 , Jonder Morais 3 , Jefferson Bettini 4 , Mateus Borba Cardoso 1, 2
Langmuir ( IF 3.7 ) Pub Date : 2020-08-07 , DOI: 10.1021/acs.langmuir.0c01545 Francine Ramos Scheffer 1, 2 , Camila Pedroso Silveira 1 , Jonder Morais 3 , Jefferson Bettini 4 , Mateus Borba Cardoso 1, 2
Affiliation
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Zwitterionic molecules are known to resist nonspecific protein adsorption and have been proposed as an alternative to the widely used polyethylene glycol. Recently, zwitterionic-like nanoparticles were created from the coimmobilization of positive and negative ligands, resulting in surfaces that also prevent protein corona formation while keeping available sites for bioconjugation. However, it is unclear if they are able to keep their original properties when immersed in biological environments while retaining a toxicity-free profile, indispensable features before considering these structures for clinics. Herein, we obtained optimized zwitterionic-like silica nanoparticles from the functionalization with varying ratios of THPMP and DETAPTMS organosilanes and investigated their behavior in realistic biological milieu. The generated zwitterionic-like particle was able to resist single-protein adsorption, while the interaction with a myriad of serum proteins led to significant loss of colloidal stability. Moreover, the zwitterionic particles presented poor hemocompatibility, causing considerable disruption of red blood cells. Our findings suggest that the exposure of ionic groups allows these structures to directly engage with the environment and that electrostatic neutrality is not enough to grant low-fouling and stealth properties.
中文翻译：
定制伪两性离子双官能化二氧化硅纳米粒子：从胶体稳定性到生物相互作用。
已知两性离子分子可抵抗非特异性蛋白质的吸附，并已提出将其作为广泛使用的聚乙二醇的替代物。近来，通过正和负配体的共固定化产生了两性离子状纳米颗粒，其表面还可以防止蛋白质电晕的形成，同时保留可用的生物缀合位点。然而，目前尚不清楚它们在浸入生物环境中时是否能够保持其原有特性，同时保持无毒特性，这是在考虑将这些结构用于临床之前的必不可少的特征。在本文中，我们从具有不同比例的THPMP和DETAPTMS有机硅烷的官能化中获得了优化的两性离子状二氧化硅纳米粒子，并研究了它们在现实生物环境中的行为。生成的两性离子样颗粒能够抵抗单蛋白吸附，而与无数种血清蛋白的相互作用导致胶体稳定性显着下降。而且，两性离子颗粒表现出差的血液相容性，引起红细胞的大量破坏。我们的发现表明，离子基团的暴露使这些结构直接与环境接触，并且静电中性不足以提供低污染和隐身性能。
更新日期：2020-09-15
中文翻译：
定制伪两性离子双官能化二氧化硅纳米粒子：从胶体稳定性到生物相互作用。
已知两性离子分子可抵抗非特异性蛋白质的吸附，并已提出将其作为广泛使用的聚乙二醇的替代物。近来，通过正和负配体的共固定化产生了两性离子状纳米颗粒，其表面还可以防止蛋白质电晕的形成，同时保留可用的生物缀合位点。然而，目前尚不清楚它们在浸入生物环境中时是否能够保持其原有特性，同时保持无毒特性，这是在考虑将这些结构用于临床之前的必不可少的特征。在本文中，我们从具有不同比例的THPMP和DETAPTMS有机硅烷的官能化中获得了优化的两性离子状二氧化硅纳米粒子，并研究了它们在现实生物环境中的行为。生成的两性离子样颗粒能够抵抗单蛋白吸附，而与无数种血清蛋白的相互作用导致胶体稳定性显着下降。而且，两性离子颗粒表现出差的血液相容性，引起红细胞的大量破坏。我们的发现表明，离子基团的暴露使这些结构直接与环境接触，并且静电中性不足以提供低污染和隐身性能。
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