Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus, and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14^Hb is a dominant major resistance gene on chromosome 6HS, originating from barley's secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n=7,500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14^Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.

中文翻译：

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野生大麦黄花叶病相对抗性基因Rym14Hb的高分辨率定位

大麦黄花叶病是由大麦黄花叶病毒和大麦轻度花叶病毒引起的，并导致中欧和东亚大麦（大麦）的严重减产。大麦育种中使用了几种抗性基因座。但是，已知有抗药性病毒株的病例，引起了对那些基因的持久性的担忧。Rym14 ^Hb是6HS染色体上的一个主要的主要抗性基因，起源于大麦的次要基因库野生亲缘球茎大麦。这样，抵抗机制可以代表非宿主抵抗的情况，这可以增强其耐久性。易感的大麦品种和抗药性球茎烟草渗入品系杂交产生大量的F2作图种群（n = 7,500），以补偿与种内大麦杂交相比重组率降低十倍的情况。高通量基因分型后，将Rym14 ^Hb基因座分配给6HS染色体上2Mbp的端粒间隔。在这项研究中开发的共分离标记物可用于以最小的连锁阻力将标记物渗入大麦精英种质。