Dorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced all of DE-3's processes. Further, we traced back the processes of all of DE-3's presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all of the behaviors were widely distributed over DE-3's branches, yet that functional clusters were different during (fictive) swimming vs. crawling.

中文翻译：

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多功能运动神经元及其周围电路的解剖和活动模式

药用水ech中的背侧兴奋运动神经元DE-3在三种不同的行为中起着三种非常不同的动力学作用。在不重新连接其解剖学连接性的情况下，运动神经元如何动态切换角色以在各种行为中扮演适当的角色？我们以前使用电压敏感染料成像来记录（虚拟）游泳，爬行和局部弯曲逃逸期间水during节神经节中的DE-3和大多数其他神经元（Tomina和Wagenaar，2017年）。在这里，我们重复了该实验，然后使用串行电子显微镜对同一神经节进行了重新成像，并追踪了DE-3的所有过程。此外，我们追溯了所有DE-3突触前伴侣的过程至其各自的躯体。这使我们能够分析电路解剖与其维持的活动模式之间的关系。