当前位置: X-MOL 学术bioRxiv. Genom. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Photomap of the polythenic chromosomes of Drosophila malerkotliana and in situ mapping of the Hsp83 locus
bioRxiv - Genomics Pub Date : 2020-08-06 , DOI: 10.1101/2020.08.05.238600
Pierre Teodósio Félix , José Ferreira dos Santos

A photomap of the polythenic chromosomes of D. malerkotliana was constructed to facilitate the identification of the chromosomal arms and their sections, in order to allow the identification of the breaking points of inversions and the location of the bands marked by in situ hybridization. The photomap included the six chromosome arms corresponding to pairs I (chromosome X), II and III, excluding pair IV and Y chromosome, because they were not visualized in the material examined, probably due to their small size. Through the in situ hybridization technique with the use of a biotined probe of a fragment of the D. melanogaster gene, the Hsp83 locus of D. malerkotliana was mapped. The probe hybridized with a frequency of 70% in section 98 of the IIIR chromosome. This is the first mapped gene in the species, and indicates that possibly the IIIR arm of D. malerkotliana corresponds to the IIIL arm of D. melanogaster, where the Hsp83 gene was located.

中文翻译:

果蝇多染色体染色体的Photomap和Hsp83基因座的原位定位

构造了D. malerkotliana的多条染色体的照片,以方便鉴定染色体臂及其部分,以便鉴定倒转的断裂点和通过原位杂交标记的条带的位置。该照片图包括对应于对I(染色体X),II和III的六个染色体臂,但不包括对IV和Y染色体对,因为它们可能在体积较小的情况下在所检查的材料中看不到。通过原位杂交技术,使用生物素化的D. melanogaster基因片段的探针,绘制了D. malerkotliana的Hsp83基因座。探针在IIIR染色体第98条中以70%的频率杂交。这是该物种中第一个定位的基因,
更新日期:2020-08-08
down
wechat
bug