Cryo-correlative light and electron microscopy (CLEM) is a technique that uses the spatiotemporal cues from fluorescence light microscopy (FLM) to investigate the high-resolution ultrastructure of biological samples by cryo-electron microscopy (cryo-EM). Cryo-CLEM provides advantages for identifying and distinguishing fluorescently labeled proteins, macromolecular complexes, and organelles from the cellular environment. Challenges remain on how correlation workflows and software tools are implemented on different microscope platforms to support microscopy-driven structural studies. Here, we present an open-source desktop application tool, CorRelator, to bridge between cryo-FLM and cryo-EM/ET data collection instruments. CorRelator was designed to be flexible for both on-the-fly and post-acquisition correlation schemes. The CorRelator workflow is easily adapted to any fluorescence and transmission electron microscope (TEM) system configuration. CorRelator was benchmarked under cryogenic and ambient temperature conditions using several FLM and TEM instruments, demonstrating that CorRelator is a rapid and efficient application for image and position registration in CLEM studies. CorRelator is a cross-platform software featuring an intuitive Graphical User Interface (GUI) that guides the user through the correlation process. CorRelator source code is available at: https://github.com/wright-cemrc-projects/corr.

中文翻译：

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CorRelator：交互式且灵活的工具包，用于高精度冷冻相关的光和电子显微镜

低温相关光电子显微镜（CLEM）是一种使用荧光显微镜（FLM）的时空线索通过冷冻电子显微镜（cryo-EM）研究生物样品的高分辨率超微结构的技术。低温CLEM提供了从细胞环境中识别和区分荧光标记的蛋白质，高分子复合物和细胞器的优势。如何在不同的显微镜平台上实施相关工作流和软件工具以支持显微镜驱动的结构研究仍然存在挑战。在这里，我们提供了一个开源桌面应用程序工具CorRelator，以在cryo-FLM和cryo-EM / ET数据收集工具之间架起桥梁。CorRelator设计为在运行中和采集后相关方案方面都具有灵活性。CorRelator工作流程很容易适应任何荧光和透射电子显微镜（TEM）系统配置。使用几种FLM和TEM仪器在低温和环境温度条件下对CorRelator进行了基准测试，表明CorRelator是CLEM研究中图像和位置配准的快速有效应用。CorRelator是一个跨平台软件，具有直观的图形用户界面（GUI），可指导用户完成相关过程。可在以下位置找到CorRelator源代码：https：//github.com/wright-cemrc-projects/corr。证明CorRelator是CLEM研究中图像和位置配准的快速而有效的应用程序。CorRelator是一个跨平台软件，具有直观的图形用户界面（GUI），可指导用户完成相关过程。可在以下位置找到CorRelator源代码：https：//github.com/wright-cemrc-projects/corr。证明CorRelator是CLEM研究中图像和位置配准的快速而有效的应用程序。CorRelator是一个跨平台软件，具有直观的图形用户界面（GUI），可指导用户完成相关过程。可在以下位置获得CorRelator源代码：https：//github.com/wright-cemrc-projects/corr。