Long-chain polyunsaturated fatty acids (LC-PUFAs), including EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) are important nutritional ingredients in fish feed. So far, fish oil has been used as a main source of LC-PUFAs; however, the limited global supply of fish oil is not able to meet the demand of the growing aquaculture sector. Hence, sustainability of aquaculture industry could be supported by searching alternative sources of these compounds. Marine microorganisms represent a sustainable and stable supply source of LC-PUFAs. A collection of 209 bacterial isolates obtained from sediment samples recovered in the Mediterranean Sea was screened in order to select new LC-PUFAs producers. Among 95 putative producers selected based on colourimetric screening, 31 quickly growing were selected for further studies. The detection of LC-PUFAs was confirmed from 15 isolates belonging to the genera Marinobacter, Halomonas and Thalassospira by GC-FID analysis. Among them, the isolate Marinobacter sp. G16.20 was found to be a potentially high LC-PUFA producer exhibiting relatively high levels of DHA in particular (maximum productivity of 1.85 ± 0.371 mg/g, representing 45.89% of the total fatty acids detected and identified). Microorganisms belonging to the genera reported in this study showed biotechnological traits interesting for their potential future application in aquaculture.

中文翻译：

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地中海细菌是长链多不饱和脂肪酸的潜在来源。

长链多不饱和脂肪酸（LC-PUFA），包括EPA（二十碳五烯酸）和DHA（二十二碳六烯酸）是鱼类饲料中重要的营养成分。到目前为止，鱼油已被用作LC-PUFA的主要来源。但是，全球鱼油供应有限，无法满足不断增长的水产养殖部门的需求。因此，可以通过寻找这些化合物的替代来源来支持水产养殖业的可持续性。海洋微生物代表着LC-PUFA的可持续和稳定的供应来源。筛选了从地中海中回收的沉积物样品中获得的209种细菌分离株，以选择新的LC-PUFAs生产商。在基于比色筛选选择的95个推定生产者中，选择了31个快速生长的生产者进行进一步研究。通过GC-FID分析对Marinobacter，Haloomonas和Thalassospira进行分析。其中，分离株Marinobacter sp。发现G16.20是潜在的高LC-PUFA生产商，尤其表现出相对较高的DHA水平（最大生产率为1.85±0.371 mg / g，占所检测和鉴定的总脂肪酸的45.89％）。这项研究中报道的属微生物显示出其在水产养殖中的潜在潜在应用所感兴趣的生物技术特征。