Our work includes the study of synergistic efficiency of turmeric crude extracts and electrical pulses on breast cancer cell line, MCF-7 (an Estrogen Receptor alpha positive, weakly invasive, luminal epithelial-like). We assessed the bioactive compounds present in this extract by qualitative phytochemical screening tests. The antioxidant, phenolic and anticancer activity of untreated and pulsed extracts were analyzed quantitatively. Here, ethanolic turmeric crude extracts were extracted by using conventional method and are subjected to varying electric field from 2.5 to 7.5 kV/cm with constant pulse interval and pulse duration as 100 ms and 100 µs, respectively, by using ECM830 Electroporator. Effect of Pulsed Electric Field (PEF) on antioxidant, phenolic and anticancer activity of turmeric extract was studied and compared with the untreated extract. Antioxidant and phenolic activity were obtained by using DPPH assay and Folin–Ciocalteu Method respectively, in which the PEF treated extracts showed significantly higher activity than the untreated extracts. Electric pulses of high intensity, low duration (EP1 = 200 V/cm, 100 µs, 8 pulses) and low intensity high duration (EP2 = 500 V/cm, 20 ms, 8 pulses) are used in this study. Cell viabilities were measured after 24, 48 and 72 h of treatment. The results indicate cell viabilities of 19.41% (raw extract), 14.75% (pulsed extract), 13.28% (pulsed extract + EP1) and 9.982% (Pulsed extract + EP2) after 72 h of treatment for 1000 µg/ml concentration. This shows the potential of the synergistic effect of electric pulses+pulsed turmeric extracts as it achieved more cell death and hence, this treatment can be used as an effective treatment to treat breast cancer without any side effects.

我们的工作包括研究姜黄粗提物和电脉冲对乳腺癌细胞系 MCF-7（一种雌激素受体 α 阳性、弱侵入性、管腔上皮样）的协同效率。我们通过定性植物化学筛选试验评估了该提取物中存在的生物活性化合物。定量分析了未处理和脉冲提取物的抗氧化、酚类和抗癌活性。此处，使用 ECM830 电穿孔仪，使用常规方法提取乙醇姜黄粗提物，并使其受到 2.5 至 7.5 kV/cm 的变化电场，脉冲间隔和脉冲持续时间分别为 100 ms 和 100 µs。脉冲电场（PEF）对抗氧化的影响，研究了姜黄提取物的酚类和抗癌活性，并与未处理的提取物进行了比较。分别使用 DPPH 测定和 Folin–Ciocalteu 方法获得抗氧化和酚类活性，其中 PEF 处理的提取物显示出比未处理的提取物显着更高的活性。本研究使用高强度、低持续时间（EP1 = 200 V/cm、100 µs、8 个脉冲）和低强度高持续时间（EP2 = 500 V/cm、20 ms、8 个脉冲）的电脉冲。在处理 24、48 和 72 小时后测量细胞活力。结果表明，在 1000 µg/ml 浓度处理 72 小时后，细胞活力为 19.41%（原始提取物）、14.75%（脉冲提取物）、13.28%（脉冲提取物 + EP1）和 9.982%（脉冲提取物 + EP2）。