Abstract

Ethanolic (EB) extract and hexanic (SH) and hydromethanolic (SEM) sub-extracts of Humulus lupulus leaves were submitted to cytotoxicity evaluation and to phytochemical methods. The effect of EB and SEM on cellular cycle was evaluated by propidium iodide method and the phases were quantified through flow cytometry. The cytotoxicity assessment was done using T24 and MRC5 cells, with EB and SEM (25–1200 µg/mL). By means of UPLC-DAD-MS/MS data were identified the flavonoids astragaline, nicotiflorin, kaempferol-7-O-rutinoside, robinin, hyperin, rutin, quercetin-7-O-rutinoside and manghaslin. EB (800 µg/mL) and SEM (1200 µg/mL) reduces the T24 cell viability. These extracts at 25 µg/mL stimulate the growth of MRC5 cells, evidencing a selective cytotoxicity. After 24 h of the treatment with extracts was not observed cycle arrest of T24 cells. The bioactivity prediction of the flavonoids was evaluated in silico through in house Active-IT software and PASSonline which indicated potential activity as antitumoral, cytotoxic, anti-inflammatory, antiparasitic, antimicrobial, antiviral and others.

摘要

将葎草叶的乙醇 (EB) 提取物和己酸 (SH) 和氢甲醇 (SEM) 亚提取物进行细胞毒性评估和植物化学方法。EB 和 SEM 对细胞周期的影响通过碘化丙啶法评估，并通过流式细胞术定量。使用 T24 和 MRC5 细胞进行细胞毒性评估，EB 和 SEM (25–1200 µg/mL)。通过 UPLC-DAD-MS/MS 数据鉴定了黄酮类化合物黄芪甲苷、烟叶苷、山奈酚-7- O-芸香苷、知更苷、金丝桃苷、芦丁、槲皮素-7- O-芸香苷和曼加林。EB (800 µg/mL) 和 SEM (1200 µg/mL) 降低了 T24 细胞的活力。这些浓度为 25 µg/mL 的提取物可刺激 MRC5 细胞的生长，证明具有选择性细胞毒性。用提取物处理 24 小时后，未观察到 T24 细胞的循环停滞。通过内部Active-IT 软件和 PASSonline在计算机上对黄酮类化合物的生物活性预测进行了评估，该软件表明其具有抗肿瘤、细胞毒性、抗炎、抗寄生虫、抗菌、抗病毒等潜在活性。