Abstract

The leaves of P. edulis were subjected to physicochemical analysis, such as ion content, extractives, and structural molecules. The hexanic, ethanolic and ethyl acetate extracts were submitted to phytochemical analyzes by GC-MS, HPLC-MS, and spectrophotometry. In addition, antioxidant (DPPH, ABTS and TAA methods) potential, antimicrobial (MIC method) action, cytotoxicity and immunostimulant activity (flow cytometry analysis) were performed. The extracts showed a moderate antioxidant capacity and revealed the presence of several metabolites, mainly phenols, such as caffeic acid, p-coumaric acid and luteolin. The ethyl acetate and ethanolic extracts showed antifungal activity. In addition, the extracts did not affect splenocytes viability at 12.5 μg/mL and promoted the production of IL-6, IL-10, IL-17 and TNF-α cytokines. P. edulis extracts showed antifungal and antioxidant activity and were able to induce immunostimulatory action in splenocyte cultures in vitro.

摘要

P. edulis的叶子进行理化分析，如离子含量、提取物和结构分子。通过 GC-MS、HPLC-MS 和分光光度法对己酸、乙醇和乙酸乙酯提取物进行植物化学分析。此外，还进行了抗氧化剂（DPPH、ABTS 和 TAA 方法）潜力、抗菌（MIC 方法）作用、细胞毒性和免疫刺激活性（流式细胞术分析）。提取物显示出中等的抗氧化能力，并揭示了几种代谢物的存在，主要是酚类，如咖啡酸、对香豆酸和木犀草素。乙酸乙酯和乙醇提取物显示出抗真菌活性。此外，提取物在 12.5 μg/mL 时不影响脾细胞活力，并促进 IL-6、IL-10、IL-17 和 TNF-α 细胞因子的产生。香菇提取物显示出抗真菌和抗氧化活性，并且能够在体外脾细胞培养物中诱导免疫刺激作用。