A universal procedure of modeling chromatography separation, based on the plate model, has been developed. On each plate, the equilibrium between the analyte in the mobile and stationary phases is established. This equilibrium may be described by the concentration-dependent partition coefficient. The establishment of the equilibrium on each plate is followed by the displacement of the analyte in the mobile phase by one plate, establishment of new equilibrium, etc. The result is a series of elution curves with positions of maxima dependent on injected mass of the polymer. The procedure has been tested on modeling concentration effects on the basis of dependences of the partition coefficient on local concentrations on each plate. By comparison with the experiment, the slope of the concentration dependence of the partition coefficient of polystyrene in benzene was estimated. It is in qualitative agreement with the literature data obtained by a computer simulation of chromatography separation of polymers in good solvents.

已经开发了一种基于平板模型对色谱分离进行建模的通用程序。在每个板上，在流动相和固定相之间建立了分析物之间的平衡。该平衡可以通过浓度依赖性分配系数来描述。在每个板上建立平衡，然后是在一个板上移动分析物在流动相中的位置，建立新的平衡，等等。结果是一系列洗脱曲线，其最大值取决于聚合物的注入质量。在分配系数对每个板上局部浓度的依赖性的基础上，对该程序进行了建模浓度效应的测试。与实验比较 估算了苯中聚苯乙烯分配系数的浓度依赖性斜率。它与通过计算机模拟在良溶剂中聚合物的色谱分离获得的文献数据在质量上相符。