ABSTRACT Pepper wilt, caused by Fusarium oxysporum, is an important disease of pepper in many regions of the world. A phosphate solubilising Trichoderma strain MHT1134 was selected from 76 isolates from the pepper rhizosphere as a potential biological control agent against wilt disease caused by F. oxysporum. The inhibitory rate of MHT1134 against F. oxysporum was 81.80%. MHT1134 was identified as Trichoderma hamatum based on morphological features, ITS rDNA and tef-1α sequences. It showed antagonistic effects against a number of fungal pathogens, including Phytophthora capsici, Colletotrichum capsici, Verticillium dahliae, Verticillium albo-atrum, Botryosphaeria dothidea, Pyricularia grisea and Phoma lingam. The fungistatic capability of strain MHT1134 was evaluated, and its sterilised and non-sterilised fermentation broths were effectively suppressed pathogen’s hyphal growth, with inhibitory effects reaching 50.71% and 74.01%, respectively. Moreover, T. hamatum MHT1134 controlled pepper wilt at a 60.61% efficiency level, and applications significantly increased plant heights, root lengths, fresh and dry weights of pepper in pot experiments. The field experiments showed that strain MHT1134 inhibited pepper Fusarium wilt, with a control effect that was only slightly less than that of the chemical hymexazol, and significantly increased pepper yield. Thus, MHT1134 is proposed as a potential biocontrol fungus and further studies will focus on its biocontrol mechanisms.

中文翻译：

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Trichoderma hamatum MHT1134促进辣椒生长和枯萎病生防

摘要 辣椒枯萎病是由尖孢镰刀菌引起的，是世界许多地区辣椒的重要病害。从辣椒根际的 76 株分离物中选出了一种增溶磷酸盐的木霉菌株 MHT1134，作为对抗由尖孢镰刀菌引起的枯萎病的潜在生物防治剂。MHT1134对尖孢镰刀菌的抑制率为81.80%。基于形态特征、ITS rDNA 和 tef-1α 序列，MHT1134 被鉴定为Trichoderma hamatum。它对许多真菌病原体显示出拮抗作用，包括辣椒疫霉、辣椒炭疽病菌、大丽轮枝菌、白轮枝菌、Botryosphaeria dothidea、稻瘟病菌和 Phoma lingam。评价菌株 MHT1134 的抑菌能力，其灭菌和非灭菌发酵液均能有效抑制病原菌菌丝生长，抑制效果分别达到50.71%和74.01%。此外，T. hamatum MHT1134 以 60.61% 的效率水平控制辣椒枯萎，在盆栽实验中，应用显着增加了辣椒的株高、根长、鲜重和干重。田间试验表明，菌株MHT1134对辣椒枯萎病有抑制作用，防治效果仅略低于化学杀虫剂，显着提高了辣椒产量。因此，MHT1134 被提议作为一种潜在的生物防治真菌，进一步的研究将集中在其生物防治机制上。hamatum MHT1134 以 60.61% 的效率水平控制辣椒枯萎，并且在盆栽实验中应用显着增加了辣椒的株高、根长、鲜重和干重。田间试验表明，菌株MHT1134对辣椒枯萎病有抑制作用，防治效果仅略低于化学杀虫剂，显着提高了辣椒产量。因此，MHT1134 被提议作为一种潜在的生物防治真菌，进一步的研究将集中在其生物防治机制上。hamatum MHT1134 以 60.61% 的效率水平控制辣椒枯萎，并且在盆栽实验中应用显着增加了辣椒的株高、根长、鲜重和干重。田间试验表明，菌株MHT1134对辣椒枯萎病有抑制作用，防治效果仅略低于化学杀虫剂，显着提高了辣椒产量。因此，MHT1134 被提议作为一种潜在的生物防治真菌，进一步的研究将集中在其生物防治机制上。并显着提高辣椒产量。因此，MHT1134 被提议作为一种潜在的生物防治真菌，进一步的研究将集中在其生物防治机制上。并显着提高辣椒产量。因此，MHT1134 被提议作为一种潜在的生物防治真菌，进一步的研究将集中在其生物防治机制上。