Background A wide range of human in vitro methods have been developed and there is considerable interest in the potential of these studies to address questions related to clinical (human) use of drugs, and the pathobiology of tumours. This requires agreement on how to assess the strength of evidence available (i.e., quality and quantity) and the human-relevance of such studies. The SAToRI-BTR (Systematic Approach To Review of in vitro methods in Brain Tumour Research) project seeks to identify relevant appraisal criteria to aid planning and/or evaluation of brain tumour studies using in vitro methods. Objectives To identify criteria for evaluation of quality and human relevance of in vitro brain tumour studies; to assess the general acceptability of such criteria to senior scientists working within the field. Methods Stage one involved identification of potential criteria for evaluation of in vitro studies through: (1) an international survey of brain tumour researchers; (2) interviews with scientists, clinicians, regulators, and journal editors; (3) analysis of relevant reports, documents, and published studies. Through content analysis of findings, an initial list of criteria for quality appraisal of in vitro studies of brain tumours was developed. Stage two involved review of the criteria by an expert panel (Delphi process). Results Results of stage one indicated that methods for and quality of review of in vitro studies are highly variable, and that improved reporting standards are needed. 129 preliminary criteria were identified; duplicate and highly context-specific items were removed, resulting in 48 criteria for review by the expert (Delphi) panel. 37 criteria reached agreement, resulting in a provisional checklist for appraisal of in vitro studies in brain tumour research. Conclusion Through a systematic process of collating assessment criteria and subjecting these to expert review, SAToRI-BTR has resulted in preliminary guidance for appraisal of in vitro brain tumour studies. Further development of this guidance, including investigating strategies for adaptation and dissemination across different sub-fields of brain tumour research, as well as the wider in vitro field, is planned.

中文翻译：

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脑肿瘤研究体外方法审查的系统方法 (SAToRI-BTR)：制定评估质量和人类相关性的初步清单

背景 已经开发了广泛的人类体外方法，并且对这些研究解决与临床（人类）使用药物和肿瘤病理生物学相关的问题的潜力具有相当大的兴趣。这需要就如何评估可用证据的强度（即质量和数量）以及此类研究的人类相关性达成一致。SAToRI-BTR（脑肿瘤研究中体外方法审查的系统方法）项目旨在确定相关的评估标准，以帮助规划和/或评估使用体外方法的脑肿瘤研究。目的 确定评估体外脑肿瘤研究质量和人类相关性的标准；评估这些标准对在该领域工作的资深科学家的普遍接受程度。方法 第一阶段涉及通过以下方式确定评估体外研究的潜在标准：（1）对脑肿瘤研究人员的国际调查；(2) 采访科学家、临床医生、监管机构和期刊编辑；(3) 分析相关报告、文件和已发表的研究。通过对研究结果的内容分析，制定了脑肿瘤体外研究质量评价标准的初步清单。第二阶段涉及由专家小组审查标准（德尔福过程）。结果 第一阶段的结果表明，体外研究的审查方法和质量存在很大差异，需要改进报告标准。确定了 129 项初步标准；删除了重复且高度特定于上下文的项目，产生了 48 条标准供专家 (Delphi) 小组审查。37 项标准达成一致，形成了用于评估脑肿瘤研究体外研究的临时清单。结论 通过对评估标准的系统整理和专家评审，SAToRI-BTR 为体外脑肿瘤研究的评估提供了初步指导。计划进一步发展本指南，包括研究跨脑肿瘤研究的不同子领域以及更广泛的体外领域的适应和传播策略。SAToRI-BTR 为体外脑肿瘤研究的评估提供了初步指导。计划进一步发展本指南，包括研究跨脑肿瘤研究的不同子领域以及更广泛的体外领域的适应和传播策略。SAToRI-BTR 为体外脑肿瘤研究的评估提供了初步指导。计划进一步发展本指南，包括研究跨脑肿瘤研究的不同子领域以及更广泛的体外领域的适应和传播策略。