Abstract Objective This study was designed to explore the biological significance of myosin light chain 6B (MYL6B) in rectal adenocarcinoma. Methods Profiles on the Oncomine dataset, GEPIA website, and UALCAN-TCGA database were searched to assess the MYL6B expression level in rectal adenocarcinoma tissues and normal tissues. After MYL6B knockdown using siRNA strategy, cell counting kit-8 (CCK-8) and transwell assays were conducted to measure cell proliferation, migration and invasion, respectively. Flow cytometry analysis was conducted to assess cell apoptosis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot were performed to detect the expression level of mRNAs and proteins. Results The data showed that overexpression of MYL6B was observed in rectal adenocarcinoma tissues and correlated with a poor prognosis of patients. Functional in vitro experiments revealed that MYL6B knockdown could inhibit proliferation, migration, and invasion of rectal adenocarcinoma cells, while promote cell apoptosis. Moreover, western blot analysis suggested that increased expression of E-cadherin and decreased expression of N-cadherin and Vimentin were induced by si-MYL6B. Conclusion In summary, this study elaborated on the promoting effect of MYL6B in rectal adenocarcinoma progression, thus providing novel insight for strategies of clinical diagnosis and drug application in the future clinical study.

中文翻译：

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MYL6B 通过 EMT 过程驱动直肠腺癌的增殖、侵袭和迁移能力

摘要 目的本研究旨在探讨肌球蛋白轻链6B（MYL6B）在直肠腺癌中的生物学意义。方法检索 Oncomine 数据集、GEPIA 网站和 UALCAN-TCGA 数据库的资料，以评估直肠腺癌组织和正常组织中 MYL6B 的表达水平。在使用 siRNA 策略敲低 MYL6B 后，进行细胞计数 kit-8 (CCK-8) 和 transwell 测定以分别测量细胞增殖、迁移和侵袭。进行流式细胞术分析以评估细胞凋亡。进行定量逆转录聚合酶链反应（qRT-PCR）和蛋白质印迹以检测mRNA和蛋白质的表达水平。结果数据显示直肠腺癌组织中存在MYL6B过表达，与患者预后不良相关。体外功能实验表明，敲低MYL6B可以抑制直肠腺癌细胞的增殖、迁移和侵袭，同时促进细胞凋亡。此外，蛋白质印迹分析表明，si-MYL6B 可诱导 E-cadherin 表达增加而 N-cadherin 和 Vimentin 表达减少。结论综上所述，本研究阐述了MYL6B在直肠腺癌进展中的促进作用，为今后临床研究的临床诊断策略和药物应用提供了新的思路。并侵袭直肠腺癌细胞，同时促进细胞凋亡。此外，蛋白质印迹分析表明，si-MYL6B 可诱导 E-cadherin 表达增加而 N-cadherin 和 Vimentin 表达减少。结论综上所述，本研究阐述了MYL6B在直肠腺癌进展中的促进作用，为今后临床研究的临床诊断策略和药物应用提供了新的思路。并侵袭直肠腺癌细胞，同时促进细胞凋亡。此外，蛋白质印迹分析表明，si-MYL6B 可诱导 E-cadherin 表达增加而 N-cadherin 和 Vimentin 表达减少。结论综上所述，本研究阐述了MYL6B在直肠腺癌进展中的促进作用，为今后临床研究的临床诊断策略和药物应用提供了新的思路。