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Antibiogram Signatures of Some Enterobacteria Recovered from Irrigation Water and Agricultural Soil in two District Municipalities of South Africa.
Microorganisms ( IF 4.5 ) Pub Date : 2020-08-07 , DOI: 10.3390/microorganisms8081206
Chidozie Declan Iwu 1, 2 , Erika M du Plessis 3 , Lise Korsten 3 , Nolonwabo Nontongana 1, 2 , Anthony Ifeanyi Okoh 1, 2
Affiliation  

This study was undertaken to evaluate the antibiogram fingerprints of some Enterobacteria recovered from irrigation water and agricultural soil in two District Municipalities of the Eastern Cape Province, South Africa using standard culture-based and molecular methods. The prevalent resistance patterns in the isolates follow the order: Salmonella enterica serovar Typhimurium [tetracycline (92.3%), ampicillin (69.2%)]; Enterobacter cloacae [amoxicillin/clavulanic acid (77.6%), ampicillin (84.5%), cefuroxime (81.0%), nitrofurantoin (81%), and tetracycline (80.3%)]; Klebsiella pneumoniae [amoxicillin/clavulanic acid (80.6%), ampicillin (88.9%), and cefuroxime (61.1%)]; and Klebsiella oxytoca [chloramphenicol (52.4%), amoxicillin/clavulanic acid (61.9%), ampicillin (61.9%), and nitrofurantoin (61.9%)]. Antibiotic resistance genes detected include tetC (86%), sulII (86%), and blaAmpC (29%) in Salmonella enterica serovar Typhimurium., tetA (23%), tetB (23%), tetC (12%), sulI (54%), sulII (54%), catII (71%), blaAmpC (86%), blaTEM (43%), and blaPER (17%) in Enterobacter cloacae., tetA (20%), tetC (20%), tetD (10%), sulI (9%), sulII (18%), FOX (11%) and CIT (11%)-type plasmid-mediated AmpC, blaTEM (11%), and blaSHV (5%) in Klebsiella pneumoniae and blaAmpC (18%) in Klebsiella oxytoca. Our findings document the occurrence of some antibiotic-resistant Enterobacteria in irrigation water and agricultural soil in Amathole and Chris Hani District Municipalities, Eastern Cape Province of South Africa, thus serving as a potential threat to food safety.

中文翻译:

从南非两个区市的灌溉水和农业土壤中回收的一些肠杆菌的抗菌素签名。

这项研究旨在评估使用标准的基于培养和分子方法从南非东开普省两个区市的灌溉水和农业土壤中回收的一些肠杆菌的抗菌素指纹图谱。分离株中普遍存在的耐药性模式依次为:肠炎沙门氏菌血清鼠伤寒[四环素(92.3%),氨苄青霉素(69.2%)];阴沟肠杆菌[阿莫西林/克拉维酸(77.6%),氨苄青霉素(84.5%),头孢呋辛(81.0%),硝基呋喃妥因(81%)和四环素(80.3%)];肺炎克雷伯菌[阿莫西林/克拉维酸(80.6%),氨苄青霉素(88.9%)和头孢呋辛(61.1%)];和产酸克雷伯菌[氯霉素(52.4%),阿莫西林/克拉维酸(61.9%),氨苄青霉素(61.9%)和硝基呋喃妥因(61.9%)]。检测到的抗生素抗性基因包括tet C(86%),sul II(86%)和bla AmpC(29%)(在肠炎沙门氏菌鼠伤寒沙门氏菌中),tet A(23%),tet B(23%),tet C(阴沟肠杆菌中的sul I(12%),sul I(54%),sul II(54%),cat II(71%),bla AmpC(86%),bla TEM(43%)和bla PER(17%)。 ,tet A(20%),tet C(20%),tet D(10%),sul I(9%),sul II(18%),FOX(11%)和CIT(11%)型质粒介导的AmpC,bla TEM(11) %),和BLA SHV(5%)在肺炎克雷伯菌BLA的AmpC(18%)在产酸克雷伯菌。我们的发现表明,在南非东开普省的Amathole和Chris Hani区市的灌溉用水和农业土壤中出现了一些抗药性肠杆菌,从而对食品安全构成潜在威胁。
更新日期:2020-08-08
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