Sequence-indexed insertional libraries in maize (Zea mays) are fundamental resources for functional genetics studies. Here, we constructed a Mutator (Mu) insertional library in the B73 inbred background designated BonnMu. A total of 1,152 Mu-tagged F₂-families were sequenced using the Mu-seq approach. We detected 225,936 genomic Mu insertion sites and 41,086 high quality germinal Mu insertions covering 16,392 of the annotated maize genes (37% of the B73v4 genome). On average, each F₂-family of the BonnMu libraries captured 37 germinal Mu insertions in genes of the Filtered Gene Set (FGS). All BonnMu insertions and phenotypic seedling photographs of Mu-tagged F₂-families can be accessed via MaizeGDB.org. Downstream examination of 137,410 somatic and germinal insertion sites revealed that 50% of the tagged genes have a single hotspot, targeted by Mu. By comparing our BonnMu (B73) data to the UniformMu (W22) library, we identified conserved insertion hotspots between different genetic backgrounds. Finally, the vast majority of BonnMu and UniformMu transposons was inserted near the transcription start site of genes. Remarkably, 75% of all BonnMu insertions were in closer proximity to the transcription start site (distance: 542 bp) than to the start codon (distance: 704 bp), which corresponds to open chromatin, especially in the 5' region of genes. Our European sequence-indexed library of Mu insertions provides an important resource for functional genetics studies of maize.

玉米 ( Zea mays )中的序列索引插入文库是功能遗传学研究的基本资源。在这里，我们在 B73 近交背景中构建了一个Mutator ( Mu ) 插入文库，指定为BonnMu。使用Mu -seq方法对总共1,152个Mu标记的F ₂ -家族进行了测序。我们检测到 225,936 个基因组Mu插入位点和 41,086 个高质量生发Mu插入，覆盖 16,392 个带注释的玉米基因（B73v4 基因组的 37%）。平均而言，BonnMu文库的每个F ₂ -家族捕获过滤基因集(FGS)的基因中的37个生发Mu插入。突变F ₂家族的所有BonnMu插入和表型幼苗照片均可通过 MaizeGDB.org 访问。对 137,410 个体细胞和生发插入位点的下游检查显示，50% 的标记基因具有Mu所针对的单一热点。通过将我们的BonnMu (B73) 数据与UniformMu (W22) 库进行比较，我们确定了不同遗传背景之间的保守插入热点。最后，绝大多数BonnMu和UniformMu转座子被插入到基因转录起始位点附近。值得注意的是，所有BonnMu插入中的 75% 距离转录起始位点（距离：542 bp）比距离起始密码子（距离：704 bp）更近，起始密码子对应于开放染色质，尤其是在基因的 5' 区域。我们的欧洲Mu插入序列索引库为玉米功能遗传学研究提供了重要资源。