Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta,FEBS Letters

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Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta
FEBS Letters ( IF 3.0 ) Pub Date : 2020-08-30 , DOI: 10.1002/1873-3468.13904
Mushtaq M Khazeem ₁ , Ian G Cowell ₁ , Lauren F Harkin ₁ , John W Casement ₂ , Caroline A Austin ₁

Affiliation

DNA topoisomerase II beta (TOP2B) has a role in transcriptional regulation. Here, to further investigate transcriptional regulation by TOP2B, we used RNA‐sequencing and real‐time PCR to analyse the differential gene expression profiles of wild‐type and two independent TOP2B‐null pre‐B Nalm‐6 cell lines, one generated by targeted insertion and the other using CRISPR‐Cas9 gene editing. We identified carbonyl reductase 1 (CBR1) among the most significantly downregulated genes in these TOP2B‐null cells. Reduced CBR1 expression was accompanied by loss of binding of the transcription factors USF2 and MAX to the CBR1 promoter. We describe possible mechanisms by which loss of TOP2B results in CBR1 downregulation. To our knowledge, this is the first report of a link between TOP2B and CBR1.

中文翻译：

羰基还原酶 1 的转录受 DNA 拓扑异构酶 II beta 调节

DNA 拓扑异构酶 II beta (TOP2B) 在转录调控中起作用。在这里，为了进一步研究 TOP2B 的转录调控，我们使用 RNA 测序和实时 PCR 来分析野生型和两个独立的 TOP2B-null pre-B Nalm-6 细胞系的差异基因表达谱，一个由靶向插入和另一个使用 CRISPR-Cas9 基因编辑。我们在这些 TOP2B-null 细胞中最显着下调的基因中鉴定出羰基还原酶 1（CBR1）。CBR1 表达降低伴随着转录因子 USF2 和 MAX 与 CBR1 启动子的结合丧失。我们描述了 TOP2B 丢失导致 CBR1 下调的可能机制。据我们所知，这是关于 TOP2B 和 CBR1 之间联系的第一份报告。

更新日期：2020-08-30

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