Airway inflammation and infections are the primary causes of damage in the airway epithelium, that lead to hypersecretion of mucus and airway hyper-responsiveness. The role of reactive oxygen species (ROS) and their components in the pathophysiological mechanisms of airway inflammation have been well-studied and emphasized for the past several decades. Rutin, a potent bioflavonoid, is well-known for its antioxidant, anti-inflammatory, especially in bronchial inflammation. However, poor solubility and rapid metabolism have led to its low bioavailability in biological systems, and hence limit its application. The present study aims to investigate the beneficial effects of rutin-loaded liquid crystalline nanoparticles (LCNs) against lipopolysaccharide (LPS) induced oxidative damage in human bronchial epithelial cell line (BEAS-2-B) cells in vitro. LPS was used to stimulate BEAS-2-B cells, causing the generation of nitric oxide (NO) and other reactive oxygen species (ROS) that had led to cellular apoptosis. The levels of NO and ROS were detected by, Griess reagent kit and dichlorodihydrofluorescein diacetate (DCFH-DA) respectively, whereas, cell apoptosis was studied by Annexin V-FITC and PI staining. The findings revealed that rutin-loaded LCNs significantly reduced NO, ROS levels and prevented apoptosis in BEAS-2B cells. The observations and findings provide a mechanistic understanding of the effectiveness of rutin-loaded LCNs in protecting the bronchial cells against airway inflammation, thus possessing a promising therapeutic option for the management of airway diseases.

气道炎症和感染是气道上皮受损的主要原因，导致粘液分泌过多和气道高反应性。在过去的几十年中，对活性氧（ROS）及其组分在气道炎症的病理生理机制中的作用进行了充分的研究和强调。芦丁是一种有效的生物类黄酮，因其抗氧化剂，抗炎作用（尤其是支气管炎症）而闻名。然而，不良的溶解度和快速的代谢导致其在生物系统中的生物利用度低，因此限制了其应用。本研究旨在调查芦丁加载液晶纳米粒子（LCNs）对脂多糖（LPS）诱导的人支气管上皮细胞系（BEAS-2-B）细胞氧化损伤的有益作用体外。LPS被用来刺激BEAS-2-B细胞，导致产生一氧化氮（NO）和其他活性氧（ROS），从而导致细胞凋亡。用Griess试剂盒和二氯二氢荧光素二乙酸酯（DCFH-DA）分别检测NO和ROS的水平，而通过Annexin V-FITC和PI染色研究细胞凋亡。研究结果表明，芦丁加载的LCNs显着降低了NO，ROS水平，并阻止了BEAS-2B细胞凋亡。这些观察和发现提供了对芦丁负载的LCNs在保护支气管细胞抵抗气道炎症中有效性的机械理解，从而为治疗气道疾病提供了有希望的治疗选择。