Exo-inulinases are versatile enzymes that have gained attention in recent years due to their ability to hydrolyze linear and branched polyfructose chains found in inulines. Agavin, a branched inulin, is found in Agave plant, the raw matter to produce tequila. Our group has isolated several microbial strains from agave bagasse, an agro-industrial residue from tequila production that increases yearly. Strain ISO3, identified as Kluyveromyces marxianus, showed a remarkable activity towards agavin, and from its fermentation liquor an inulinolytic enzyme (Inu-ISO3) was purified. The isolated enzyme is a glycosylated dimeric protein with a molecular mass of ~256 kDa, as determined by DLS and SEC. The enzyme has an isoelectric pH of 4.6 and has both inulinase and invertase activities with an I/S ratio (ratio of activity with agavin to activity with sucrose) of 1.39. The enzyme has temperature and pH optima of 50 °C and 5.5, respectively, and follows hyperbolic kinetics with agavin (k_cat of 339 ± 27 s⁻¹ and K_M of 11.8 ± 1.5 mM). The remarkable activity of Inu-ISO3 on linear and branched inulin spotlights this enzyme as a potential player in the treatment of agricultural residua for the generation of added-value products.

菊糖外切菊糖酶是通用酶，由于其水解菊糖中发现的线性和支链多聚果糖链的能力，近年来受到关注。龙舌兰植物中发现了一种分支菊粉Agavin，龙舌兰是生产龙舌兰酒的原料。我们小组从龙舌兰蔗渣中分离出了几种微生物菌株，龙舌兰蔗渣是龙舌兰酒生产中的一种农业工业残留物，并且每年都在增加。ISO3菌株，鉴定为马克斯克鲁维酵母，显示了对龙舌兰的显着活性，并从其发酵液中纯化了亚尿酸分解酶（Inu-ISO3）。分离的酶是一种糖基化的二聚体蛋白，分子量约为256 kDa，由DLS和SEC测定。该酶的等电点pH值为4.6，具有菊粉酶和蔗糖酶活性，I / S比（龙舌兰活性与蔗糖活性之比）为1.39。该酶的最适温度和最适pH分别为50°C和5.5，并遵循双曲线动力学和加拉芬（k _cat为339±27 s ^-1和K _M（11.8±1.5 mM）。Inu-ISO3在线性和支链菊粉上的卓越活性使该酶成为治疗农业残留物以产生增值产品的潜在参与者。