当前位置：
X-MOL 学术
›
Phytochemistry
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Natural variation in specialised metabolites production in the leafy vegetable spider plant (Gynandropsis gynandra L. (Briq.)) in Africa and Asia
Phytochemistry ( IF 3.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.phytochem.2020.112468 E O Dêêdi Sogbohossou 1 , Enoch G Achigan-Dako 2 , Roland Mumm 3 , Ric C H de Vos 3 , M Eric Schranz 4
Phytochemistry ( IF 3.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.phytochem.2020.112468 E O Dêêdi Sogbohossou 1 , Enoch G Achigan-Dako 2 , Roland Mumm 3 , Ric C H de Vos 3 , M Eric Schranz 4
Affiliation
|
The improvement and promotion of leafy vegetables, used for both food and medicine, benefits greatly from detailed knowledge of their health-promoting specialised metabolites. In the present study, we investigated the global metabolite variation in the leaves of 48 accessions of the leafy vegetable Gynandropsis gynandra using two complementary analytical platforms: liquid-chromatography mass spectrometry (LC-MS) for an untargeted comparison of non-volatile semi-polar metabolites and gas-chromatography mass spectrometry (GC-MS) for an untargeted comparison of volatile metabolites. Our results revealed large variation in 936 semi-polar compounds including flavonoids, terpene glycosides, glucosinolates and various phenolic compounds. Unsupervised multivariate analysis indicated the variation in levels of the semi-polar metabolites was mainly driven by geography, with accessions from both West Africa and Asia forming a group clearly separated from East African accessions. Detected volatile metabolites included various sesquiterpenes, aldehydes, ketones, and sulphur-containing isothiocyanates. Variation in these compounds was however not geographically specific, but most likely linked to the taste and aroma of the leaves. The relative abundance in glucosinolates and isothiocyanates in the leaves allowed the clustering of accessions into two main groups that could be used for further plant-herbivore interaction studies. This study revealed both the broad spectrum of phytochemicals present in Gynandropsis gynandra leaves and the substantial variation in metabolite profiles among accessions from different regions of the world. Our results provide a basis for the development of breeding programs aiming at improving the levels of specialised metabolites in this tropical leafy vegetable for increased resistance against pests and diseases and improved human health.
中文翻译：
非洲和亚洲多叶蔬菜吊兰（Gynandropsis gynandra L. (Briq.)）中特殊代谢产物的自然变异
用于食品和药物的叶菜类蔬菜的改良和推广得益于对其促进健康的特殊代谢物的详细了解。在本研究中，我们使用两个互补的分析平台：液相色谱质谱 (LC-MS)，对 48 个叶菜植物 Gynandropsis gynandra 的叶子中的整体代谢物变化进行了非靶向比较代谢物和气相色谱质谱 (GC-MS) 对挥发性代谢物进行非靶向比较。我们的结果揭示了 936 种半极性化合物的巨大变化，包括黄酮类化合物、萜糖苷、硫代葡萄糖苷和各种酚类化合物。无监督的多变量分析表明，半极性代谢物水平的变化主要受地理因素影响，来自西非和亚洲的种质形成了一个与东非种质明显分离的组。检测到的挥发性代谢物包括各种倍半萜烯、醛、酮和含硫异硫氰酸酯。然而，这些化合物的变化并非地域特定，而很可能与叶子的味道和香气有关。叶子中硫代葡萄糖苷和异硫氰酸酯的相对丰度允许将种质聚类为两个主要组，可用于进一步的植物-食草动物相互作用研究。这项研究揭示了 Gynandropsis gynandra 叶子中存在的广谱植物化学物质，以及来自世界不同地区的种质之间代谢物谱的显着差异。我们的研究结果为制定育种计划提供了基础，旨在提高这种热带叶类蔬菜中专门代谢物的水平，以增强对病虫害的抵抗力并改善人类健康。
更新日期：2020-10-01
中文翻译：
非洲和亚洲多叶蔬菜吊兰（Gynandropsis gynandra L. (Briq.)）中特殊代谢产物的自然变异
用于食品和药物的叶菜类蔬菜的改良和推广得益于对其促进健康的特殊代谢物的详细了解。在本研究中，我们使用两个互补的分析平台：液相色谱质谱 (LC-MS)，对 48 个叶菜植物 Gynandropsis gynandra 的叶子中的整体代谢物变化进行了非靶向比较代谢物和气相色谱质谱 (GC-MS) 对挥发性代谢物进行非靶向比较。我们的结果揭示了 936 种半极性化合物的巨大变化，包括黄酮类化合物、萜糖苷、硫代葡萄糖苷和各种酚类化合物。无监督的多变量分析表明，半极性代谢物水平的变化主要受地理因素影响，来自西非和亚洲的种质形成了一个与东非种质明显分离的组。检测到的挥发性代谢物包括各种倍半萜烯、醛、酮和含硫异硫氰酸酯。然而，这些化合物的变化并非地域特定，而很可能与叶子的味道和香气有关。叶子中硫代葡萄糖苷和异硫氰酸酯的相对丰度允许将种质聚类为两个主要组，可用于进一步的植物-食草动物相互作用研究。这项研究揭示了 Gynandropsis gynandra 叶子中存在的广谱植物化学物质，以及来自世界不同地区的种质之间代谢物谱的显着差异。我们的研究结果为制定育种计划提供了基础，旨在提高这种热带叶类蔬菜中专门代谢物的水平，以增强对病虫害的抵抗力并改善人类健康。
京公网安备 11010802027423号