Abstract Potential contamination sources and the enterotoxigenic profiling of Staphylococcus isolates were investigated in four goat dairy plants. Samples (n = 160) were collected from different processing points in five samplings. Staphylococcus spp. isolates were confirmed by polymerase chain reaction and genotyped using repetitive extragenic palindromic PCR (Rep-PCR). Classical (sea, seb, sec, sed) and novel (seg, seh, sei) staphylococcal enterotoxin-encoding genes were detected by PCR. seb, sec, see and sei were identified in isolates originated from raw and pasteurised milk, pre- and post-pasteurisation tanks and wall surfaces. SE-harbouring isolates were mostly identified as non-aureus staphylococci. Genotypic profiles and contamination levels indicate that the packaging machine is a critical point for staphylococcal contamination. Equipment surfaces are important contamination sources to pasteurised milk considering the indistinguishable genotypic patterns of isolates from pre- and post-pasteurisation points. Cleaning and disinfection of milk contact surfaces should be revisited to warrant quality and safety of goat milk.

中文翻译：

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设备接触面是山羊奶乳制品厂中携带肠毒素编码基因的葡萄球菌的来源

摘要 对四家山羊奶厂的潜在污染源和葡萄球菌分离株的肠毒素谱进行了调查。样品 (n = 160) 是从五个样品中的不同处理点收集的。葡萄球菌属 通过聚合酶链反应确认分离株，并使用重复基因外回文 PCR (Rep-PCR) 进行基因分型。通过PCR检测经典（sea、seb、sec、sed）和新型（seg、seh、sei）葡萄球菌肠毒素编码基因。seb、sec、see 和 sei 在源自生奶和巴氏杀菌奶、巴氏杀菌前和巴氏杀菌后的罐和墙壁表面的分离物中得到鉴定。携带 SE 的分离株大多被鉴定为非金黄色葡萄球菌。基因型谱和污染水平表明包装机是葡萄球菌污染的关键点。考虑到来自巴氏杀菌前和巴氏杀菌后点的分离物无法区分的基因型模式，设备表面是巴氏杀菌牛奶的重要污染源。应重新检查牛奶接触面的清洁和消毒，以保证山羊奶的质量和安全。