Johne’s disease is chronic, incurable disease, caused by Mycobacterium avium subsp. Paratuberculosis (MAP). Most studies in Egypt focused on incidence of the disease in cattle but few studies were reported presence of antibodies against MAP in sheep. The present study determines seroprevalence rate of MAP among sheep in four Governorates and assessed the associated risk factors to MAP-infection. The seroprevalence rate of MAP among sheep was non-significant varied between different Governorates, it was ranged between 3.75% to 12.3%. The results revealed that the seroprevalence rate of the disease was significantly increased in diarrheic sheep (11%, 95%CI: 7.2-16.2) during spring (15%, 95%CI: 8.3-25) and summer (8%, 95%CI: 4.13-13.8) seasons. Contrary, the age of sheep and contact with other ruminants like cattle or goats had non-significant effect of spreading of MAP-infection among sheep. The investigation of MAP in feces of sheep was carried out using PCR and culture to determine the efficiency of both tests. The kappa test revealed good agreement between both tests for detection of MAP. The obtained finding confirms the presence of MAP among sheep in Egypt. So, the appropriate control measures should be taken to reduce spreading of the disease among ruminant and reduce its economic losses.

约翰尼氏病是由鸟分枝杆菌亚种引起的慢性，不治之症。副结核病（地图）。埃及的大多数研究都集中在牛的发病率上，但很少有研究报道绵羊中存在针对MAP的抗体。本研究确定了四个省绵羊之间的MAP血清阳性率，并评估了MAP感染的相关危险因素。绵羊之间MAP的血清阳性率在各省之间没有显着差异，在3.75％至12.3％之间。结果显示，春季（15％，95％CI：8.3-25）和夏季（8％，95％）的腹泻绵羊（11％，95％CI：7.2-16.2）的疾病血清阳性率显着增加CI：4.13-13.8）季节。相反，绵羊的年龄以及与其他反刍动物如牛或山羊的接触对在绵羊之间传播MAP感染没有显着影响。使用PCR和培养进行了绵羊粪便中MAP的研究，以确定两种测试的效率。kappa测试显示两个测试之间在检测MAP方面有很好的一致性。获得的发现证实了埃及绵羊中存在MAP。因此，应采取适当的控制措施以减少该疾病在反刍动物中的传播并减少其经济损失。