Toxoplasmosis, caused by Toxoplasma gondii, is a parasitic zoonosis of crucial medical and veterinary importance. It is mainly diagnosed by serological methods which are limited by insufficient sensitivity. Therefore, it is necessary to rely on direct detection of the parasite. The present study was aimed for direct detection of the parasite DNA in the blood samples of sheep and goats using PCR targeting the B1 gene. The study was carried out in 20 small ruminant farms between 2016 and 2018 in Tebessa region, part of north-eastern Algeria, and concerned 227 and 91 aborted female sheep and goats respectively. DNA of T. gondii was detected in 35.24 % and 18.68 % blood samples of sheep and goats respectively (p < 0.001). Molecular prevalence was higher in 13−24 month old female sheep (93.33 %) than 1−12 month old female sheep (14.37 %) (p < 0.0001). While, in goats no significant difference was observed in relation to age. Female sheep that aborted between 1−60 days of gestation were found to be more infected (46.41 %) compared to females that aborted between 61−120 days of gestation (12.16 %) (p < 0.001). Whereas, female goats that aborted between 61−120 days of gestation were found to be more infested (30.77 %) compared to females that aborted between 1−60 days of gestation (16.67 %) (p < 0.001). This study revealed that small ruminants are highly infected with T. gondii, which represents a major risk for the consumer in Tebessa. Further studies are needed to improve our knowledge of the different genotypes of T. gondii infecting small ruminant population.

由弓形虫引起的弓形虫病是具有重要医学和兽医意义的寄生虫人畜共患病。它主要是通过血清学方法诊断的，而这种方法受到灵敏度不足的限制。因此，有必要依靠对寄生虫的直接检测。本研究旨在使用靶向B1基因的PCR直接检测绵羊和山羊血液样本中的寄生虫DNA。该研究于2016年至2018年期间在阿尔及利亚东北部特比萨地区的20个小反刍动物农场中进行，分别涉及227只和91只流产的雌性绵羊和山羊。弓形虫的DNA分别在绵羊和山羊的血液样本中检测到35.24％和18.68％的尿样（p <0.001）。13-24个月大的雌性绵羊的分子患病率（93.33％）高于1-12个月大的雌性绵羊（14.37％）（p <0.0001）。而在山羊中，年龄方面没有发现显着差异。与在妊娠61-120天之间流产的母羊（12.16％）相比，在妊娠1-60天之间流产的母羊被感染（46.41％）的感染率更高（p <0.001）。而在妊娠61-120天之间流产的雌性山羊被感染的比例更高（30.77％），而在妊娠1-60天之间流产的雌性山羊的感染率更高（16.67％）（p <0.001）。这项研究表明，小反刍动物被弓形虫高度感染，这对Tebessa的消费者构成了重大风险。需要进一步的研究来提高我们对感染小反刍动物群体的弓形虫不同基因型的了解。