Hypoxia can lead to adverse effects on growth, reproduction, behavioral activities and survival in fish, and is one of the most critical factors in the aquatic environment. The liver is an important target organ for reducing toxin accumulation and hypoxia in fish. In this study, silver sillago (Sillago sihama) was exposed to normoxia (dissolved oxygen, DO = 8.0 mg/L), hypoxia for 1 h (hypoxia 1 h, DO = 1.5 mg/L), hypoxia for 4 h (hypoxia 4 h, DO = 1.5 mg/L) and reoxygenation for 4 h after hypoxia 4 h (reoxygenation 4 h, DO = 8.0 mg/L). Results showed that the expression of 506, 1721, and 1230 differentially expressed genes (DEGs) (|log₂₍fold change) > 1.0| and padj < 0.05) were identified at hypoxia 1 h, hypoxia 4 h, and reoxygenation 4 h in the liver, respectively. The enrichment analysis showed that the DEGs were significantly enriched in metabolic and translation changes pathways, including mapk signaling pathway, p53 signaling pathway, fatty acid metabolism, protein export, ribosome biogenesis in eukaryotes. The DEGs of 17 genes validated the RNA-seq results by quantitative real-time PCR (qRT-PCR). This study provides a comprehensive understanding of the transcriptional changes that occur in different hypoxia and insights into the mechanisms of hypoxia adaptation of the liver in S. sihama.

缺氧可能对鱼类的生长，繁殖，行为活动和生存产生不利影响，并且是水生环境中最关键的因素之一。肝脏是减少鱼类毒素积累和缺氧的重要靶器官。在这项研究中，新罗银（Sillago sihama）暴露于常氧（溶解氧，DO = 8.0 mg / L），缺氧1 h（低氧1 h，DO = 1.5 mg / L），缺氧4 h（低氧4 h，DO = 1.5 mg / L），缺氧4 h后再充氧4 h（再充氧4 h，DO = 8.0 mg / L）。结果表明，506、1721、1230差异表达基因（DEGs）的表达（| log _2（倍数变化）> 1.0 |和padj <0.05）分别在肝脏缺氧1 h，缺氧4 h和复氧4 h时被鉴定。富集分析表明，DEGs在代谢和翻译变化途径中显着富集，包括mapk信号传导途径，p53信号传导途径，脂肪酸代谢，蛋白质输出，真核生物的核糖体生物发生。17个基因的DEG通过定量实时PCR（qRT-PCR）验证了RNA-seq结果。这项研究提供了一个全面的理解在不同的缺氧发生的转录变化，并深入了解西滨沙鼠肝脏的缺氧适应机制。