Diisononyl phthalate (DINP) as a phthalate plasticizer is widely used in daily life and production, which shows endocrine disruption effects and has several adverse effects on the normal physiological function. Here, the effects of DINP (0.1%, 0.2%, 0.5%, and 1.0%) (v/v) on the fertility, lifespan, climbing behavior, anti-starvation ability of Drosophila melanogaster and the potential modes of action were investigated. The results showed that DINP impaired fertility in a dose-dependent manner and smaller ovarian volume, lower hatching rate, and fewer offspring was observed at higher concentrations. The effect of DINP on the lifespan showed gender-specific, and mortality was increased after exposure above 0.2% DINP. The climbing ability increased at 0.1% DINP compared with the vehicle group, while it manifested a dose-dependent decrease at higher concentrations. The anti-starvation ability exhibited hormesis after short-term culture and reduced as culture time extending. By measuring the redox status (catalase (CAT) and reactive oxygen species (ROS)) of adult flies after two exposure methods, it was found that DINP induced redox instability, which may explain the above effects at the molecular level. This study provides data to support a comprehensive analysis of DINP potential toxicity and to guide its rational use and management better.

邻苯二甲酸二异壬酯（DINP）作为邻苯二甲酸酯类增塑剂已广泛应用于日常生活和生产中，显示出内分泌干扰作用，并对正常的生理功能有若干不利影响。在此，DINP（0.1％，0.2％，0.5％和1.0％）（v / v）对果蝇的生育力，寿命，攀爬行为和抗饥饿能力的影响并研究了潜在的作用方式。结果表明，DINP以剂量依赖的方式损害生育能力，在较高的浓度下观察到卵巢体积较小，孵化率较低且后代较少。DINP对寿命的影响显示出性别特异性，暴露于0.2％DINP以上后死亡率增加。与媒介物组相比，攀爬能力在0.1％DINP下增加，而在较高浓度下表现出剂量依赖性下降。短期培养后，抗饥饿能力表现出兴奋性，并随着培养时间的延长而降低。通过测量两种接触方法后成年果蝇的氧化还原状态（过氧化氢酶（CAT）和活性氧（ROS）），发现DINP引起氧化还原不稳定，这可以在分子水平上解释上述影响。