The question about the influence of the geometry of silver nanoparticle (nAg) towards toxicity in aquatic organisms is largely unanswered. The purpose of this study was to examine if different geometries of nAg could initiate biophysical stress in the soft tissues of mussels. Freshwater Dreissenna bugensis mussels were exposed for 48 h at 15 °C to 10 and 50 μg/L of ionic Ag and to 3 forms of polyvinylpyrrolidone (PVP)-coated nAg of similar size: sphere, cube and prism. At the end of the exposure period, mussels were allowed to depurate overnight and the post-mitochondrial fraction of the soft tissues were analyzed for the levels of liquid crystals (LCs), changes in the activity and fractal dimensions of pyruvate kinase-lactate dehydrogenase (PK-LDH), F-actin and protein-ubiquitin (UB) levels. The data revealed that exposure to nAg forms lead to increased formation of LCs in increasing order of intensity: prismatic > cubic > spherical nAg. The activity in PK-LDH was decreased by all forms of nAg but not by ionic Ag⁺ (as with the following effects). Fractal kinetics of the PK-LDH system revealed that the nAg forms increased the spectral dimension (sD) in increasing order: spherical > cubic > prismatic nAg. A decrease in the fractal diffusion rate (fDR) with small changes in the fractal dimension (fD) was also obtained. The levels of F-actin and protein-UB were significantly affected for most forms of nAg and followed a pattern similar to LCs levels. In conclusion, the geometry of nAg could influence the formation of LCs, alter the fractal kinetics of the PK-LDH system, F-actin levels and protein damage in the soft tissues of freshwater mussels.

关于银纳米颗粒（nAg）的几何形状对水生生物毒性的影响的问题在很大程度上尚未得到解答。这项研究的目的是检查不同几何形状的nAg是否会引发贻贝软组织中的生物物理应力。淡水臭虫贻贝在15°C下暴露48 h，暴露于10和50μg/ L的离子Ag和3种形式的类似大小的聚乙烯吡咯烷酮（PVP）涂层的nAg：球形，立方体和棱柱形。在暴露期结束时，将贻贝净化过夜，然后分析软组织的线粒体后部分的液晶（LC）水平，丙酮酸激酶-乳酸脱氢酶的活性变化和分形维数（ PK-LDH），F-肌动蛋白和蛋白质泛素（UB）水平。数据显示，暴露于nAg形式导致LC形成的强度顺序增加：棱柱形>立方>球形nAg。各种形式的nAg都会降低PK-LDH的活性，但离子Ag ⁺不会降低（具有以下效果）。PK-LDH系统的分形动力学表明，nAg形式以增加的顺序增加了光谱尺寸（sD）：球形>立方>棱柱形nAg。随着分形维数（fD）的微小变化，分形扩散率（fDR）也会降低。对于大多数形式的nAg，F-肌动蛋白和蛋白质-UB的水平均受到显着影响，并遵循类似于LCs水平的模式。总之，nAg的几何形状可能会影响LC的形成，改变PK-LDH系统的分形动力学，F-肌动蛋白水平和淡水贻贝软组织中的蛋白质损伤。